Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis

Cristina Beltrami, Marie Besnier, Saran Shantikumar, Andrew I.U. Shearn, Cha Rajakaruna, Abas Laftah, Fausto Sessa, Gaia Spinetti, Enrico Petretto, Gianni D. Angelini, Costanza Emanueli

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

The pericardial fluid (PF) is contained in the pericardial sac surrounding the heart. MicroRNA (miRNA) exchange via exosomes (endogenous nanoparticles) contributes to cell-to-cell communication. We investigated the hypotheses that the PF is enriched with miRNAs secreted by the heart and that it mediates vascular responses through exosome exchange of miRNAs. The study was developed using leftover material from aortic valve surgery. We found that in comparison with peripheral plasma, the PF contains exosomes enriched with miRNAs co-expressed in patients’ myocardium and vasculature. At a functional level, PF exosomes improved survival, proliferation, and networking of cultured endothelial cells (ECs) and restored the angiogenic capacity of ECs depleted (via Dicer silencing) of their endogenous miRNA content. Moreover, PF exosomes improved post-ischemic blood flow recovery and angiogenesis in mice. Mechanistically, (1) let-7b-5p is proangiogenic and inhibits its target gene, TGFBR1, in ECs; (2) PF exosomes transfer a functional let-7b-5p to ECs, thus reducing their TGFBR1 expression; and (3) let-7b-5p depletion in PF exosomes impairs the angiogenic response to these nanoparticles. Collectively, our data support the concept that PF exosomes orchestrate vascular repair via miRNA transfer.
Original languageEnglish
Pages (from-to)679–693
Number of pages15
JournalMolecular Therapy
Volume25
Issue number3
Early online date1 Feb 2017
DOIs
Publication statusPublished - 1 Mar 2017

Structured keywords

  • Centre for Surgical Research

Keywords

  • pericardial fluid
  • exosomes
  • microRNAs
  • let-7b
  • angiogenesis
  • ischemia
  • extracellular vesicles
  • clinical samples
  • human

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    Beltrami, C., Besnier, M., Shantikumar, S., Shearn, A. I. U., Rajakaruna, C., Laftah, A., Sessa, F., Spinetti, G., Petretto, E., Angelini, G. D., & Emanueli, C. (2017). Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis. Molecular Therapy, 25(3), 679–693. https://doi.org/10.1016/j.ymthe.2016.12.022