Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults

Isabel Leroux-Roels; Marie Van der Wielen; Froukje Kafeja; Corinne Vandermeulen; Rajeka Lazarus; Matthew D Snape; Tessa John; Christophe Carre; Nolwenn Nougarede; Stephanie Pepin; Geert Leroux-Roels; Karel Hoppenbrouwers; Andrew J Pollard; Pierre Van Damme

doi:10.1016/j.vaccine.2009.08.110

Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults

Isabel Leroux-Roels, Marie Van der Wielen, Froukje Kafeja, Corinne Vandermeulen, Rajeka Lazarus, Matthew D Snape, Tessa John, Christophe Carre, Nolwenn Nougarede, Stephanie Pepin, Geert Leroux-Roels, Karel Hoppenbrouwers, Andrew J Pollard, Pierre Van Damme

Research output: Contribution to journal › Article (Academic Journal) › peer-review

22 Citations (Scopus)

Abstract

We evaluated the humoral and cellular immunogenicity of adjuvanted and non-adjuvanted H5N1 influenza vaccine in two groups of 300 adults: aged 18-60 and >60 years in a randomized, open-label, uncontrolled phase 2 trial. Participants received two injections (D0, D21) of 7.5 microg hemagglutinin without adjuvant or 30 microg with aluminum hydroxide adjuvant. Antibody responses and cytokine secretion were assessed before and after vaccination. Excluding the 6/300 non-elderly and 47/300 elderly participants with pre-existing antibodies, geometric mean titers (dil(-1)) on D42 were higher with 30 microg+Ad and were comparable between age groups. Participants with pre-existing antibodies responded strongly to the first vaccination (GMTs in the range 147-228 on D21). Vaccination increased both Th1 and Th2 T-cell responses. The predominantly Th1 profile observed before vaccination was unaffected by vaccination. H5N1 influenza vaccine is no less immunogenic in elderly adults than in younger adults and, due to a higher proportion non-naïve elderly, immunogenicity was higher in this latter group.

Original language	English
Pages (from-to)	6918-25
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	49
DOIs	https://doi.org/10.1016/j.vaccine.2009.08.110
Publication status	Published - 16 Nov 2009

Keywords

Adjuvants, Immunologic
Adult
Aged
Aluminum Hydroxide/immunology
Antibodies, Viral/blood
Cytokines/immunology
Female
Hemagglutinin Glycoproteins, Influenza Virus/immunology
Humans
Immunity, Cellular
Immunity, Humoral
Immunization, Secondary
Influenza A Virus, H5N1 Subtype/immunology
Influenza Vaccines/immunology
Influenza, Human/immunology
Male
Middle Aged
Th1 Cells/immunology
Th2 Cells/immunology
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Leroux-Roels, I., Van der Wielen, M., Kafeja, F., Vandermeulen, C., Lazarus, R., Snape, M. D., John, T., Carre, C., Nougarede, N., Pepin, S., Leroux-Roels, G., Hoppenbrouwers, K., Pollard, A. J., & Van Damme, P. (2009). Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults. Vaccine, 27(49), 6918-25. https://doi.org/10.1016/j.vaccine.2009.08.110

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title = "Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults",

abstract = "We evaluated the humoral and cellular immunogenicity of adjuvanted and non-adjuvanted H5N1 influenza vaccine in two groups of 300 adults: aged 18-60 and >60 years in a randomized, open-label, uncontrolled phase 2 trial. Participants received two injections (D0, D21) of 7.5 microg hemagglutinin without adjuvant or 30 microg with aluminum hydroxide adjuvant. Antibody responses and cytokine secretion were assessed before and after vaccination. Excluding the 6/300 non-elderly and 47/300 elderly participants with pre-existing antibodies, geometric mean titers (dil(-1)) on D42 were higher with 30 microg+Ad and were comparable between age groups. Participants with pre-existing antibodies responded strongly to the first vaccination (GMTs in the range 147-228 on D21). Vaccination increased both Th1 and Th2 T-cell responses. The predominantly Th1 profile observed before vaccination was unaffected by vaccination. H5N1 influenza vaccine is no less immunogenic in elderly adults than in younger adults and, due to a higher proportion non-na{\"i}ve elderly, immunogenicity was higher in this latter group.",

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author = "Isabel Leroux-Roels and {Van der Wielen}, Marie and Froukje Kafeja and Corinne Vandermeulen and Rajeka Lazarus and Snape, {Matthew D} and Tessa John and Christophe Carre and Nolwenn Nougarede and Stephanie Pepin and Geert Leroux-Roels and Karel Hoppenbrouwers and Pollard, {Andrew J} and {Van Damme}, Pierre",

year = "2009",

month = nov,

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doi = "10.1016/j.vaccine.2009.08.110",

language = "English",

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Leroux-Roels, I, Van der Wielen, M, Kafeja, F, Vandermeulen, C, Lazarus, R, Snape, MD, John, T, Carre, C, Nougarede, N, Pepin, S, Leroux-Roels, G, Hoppenbrouwers, K, Pollard, AJ & Van Damme, P 2009, 'Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults', Vaccine, vol. 27, no. 49, pp. 6918-25. https://doi.org/10.1016/j.vaccine.2009.08.110

TY - JOUR

T1 - Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults

AU - Leroux-Roels, Isabel

AU - Van der Wielen, Marie

AU - Kafeja, Froukje

AU - Vandermeulen, Corinne

AU - Lazarus, Rajeka

AU - Snape, Matthew D

AU - John, Tessa

AU - Carre, Christophe

AU - Nougarede, Nolwenn

AU - Pepin, Stephanie

AU - Leroux-Roels, Geert

AU - Hoppenbrouwers, Karel

AU - Pollard, Andrew J

AU - Van Damme, Pierre

PY - 2009/11/16

Y1 - 2009/11/16

N2 - We evaluated the humoral and cellular immunogenicity of adjuvanted and non-adjuvanted H5N1 influenza vaccine in two groups of 300 adults: aged 18-60 and >60 years in a randomized, open-label, uncontrolled phase 2 trial. Participants received two injections (D0, D21) of 7.5 microg hemagglutinin without adjuvant or 30 microg with aluminum hydroxide adjuvant. Antibody responses and cytokine secretion were assessed before and after vaccination. Excluding the 6/300 non-elderly and 47/300 elderly participants with pre-existing antibodies, geometric mean titers (dil(-1)) on D42 were higher with 30 microg+Ad and were comparable between age groups. Participants with pre-existing antibodies responded strongly to the first vaccination (GMTs in the range 147-228 on D21). Vaccination increased both Th1 and Th2 T-cell responses. The predominantly Th1 profile observed before vaccination was unaffected by vaccination. H5N1 influenza vaccine is no less immunogenic in elderly adults than in younger adults and, due to a higher proportion non-naïve elderly, immunogenicity was higher in this latter group.

AB - We evaluated the humoral and cellular immunogenicity of adjuvanted and non-adjuvanted H5N1 influenza vaccine in two groups of 300 adults: aged 18-60 and >60 years in a randomized, open-label, uncontrolled phase 2 trial. Participants received two injections (D0, D21) of 7.5 microg hemagglutinin without adjuvant or 30 microg with aluminum hydroxide adjuvant. Antibody responses and cytokine secretion were assessed before and after vaccination. Excluding the 6/300 non-elderly and 47/300 elderly participants with pre-existing antibodies, geometric mean titers (dil(-1)) on D42 were higher with 30 microg+Ad and were comparable between age groups. Participants with pre-existing antibodies responded strongly to the first vaccination (GMTs in the range 147-228 on D21). Vaccination increased both Th1 and Th2 T-cell responses. The predominantly Th1 profile observed before vaccination was unaffected by vaccination. H5N1 influenza vaccine is no less immunogenic in elderly adults than in younger adults and, due to a higher proportion non-naïve elderly, immunogenicity was higher in this latter group.

KW - Adjuvants, Immunologic

KW - Adult

KW - Aged

KW - Aluminum Hydroxide/immunology

KW - Antibodies, Viral/blood

KW - Cytokines/immunology

KW - Female

KW - Hemagglutinin Glycoproteins, Influenza Virus/immunology

KW - Humans

KW - Immunity, Cellular

KW - Immunity, Humoral

KW - Immunization, Secondary

KW - Influenza A Virus, H5N1 Subtype/immunology

KW - Influenza Vaccines/immunology

KW - Influenza, Human/immunology

KW - Male

KW - Middle Aged

KW - Th1 Cells/immunology

KW - Th2 Cells/immunology

KW - Young Adult

U2 - 10.1016/j.vaccine.2009.08.110

DO - 10.1016/j.vaccine.2009.08.110

M3 - Article (Academic Journal)

C2 - 19761837

SN - 0264-410X

VL - 27

SP - 6918

EP - 6925

JO - Vaccine

JF - Vaccine

IS - 49

ER -

Humoral and cellular immune responses to split-virion H5N1 influenza vaccine in young and elderly adults

Abstract

Keywords

UN SDGs

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