Ice recrystallisation inhibition by polyols: comparison of molecular and macromolecular inhibitors and role of hydrophobic units

Robert C. Deller*, Thomas Congdon, Mohammed A. Sahid, Michael Morgan, Manu Vatish, Daniel A. Mitchell, Rebecca Notman, Matthew I. Gibson

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

33 Citations (Scopus)

Abstract

The ability of polyols to act as ice recrystallisation inhibitors (IRI), inspired by antifreeze (glyco) proteins are studied. Poly(vinyl alcohol), PVA, a known IRI active polymer was compared to a panel of mono and polysaccharides, with the aim of elucidating why some polyols are active and others show no activity. When corrected for total hydroxyl concentration all the carbohydrate-based polyols displayed near identical activity with no significant influence of molecular weight. Conversely, PVA was several orders of magnitude more active and its activity displays significant dependence on molecular-weight implying that its mechanism of action is not identical to that of carbohydrates. In a second step, the role of hydrophobicity was studied and it is observed that monosaccharide IRI activity is enhanced by alkylation. Dye-quenching assays demonstrated that PVA is able to present a hydrophobic surface without self-aggregation. Therefore, the ability to present a hydrophobic domain is hypothesised to be essential to obtain high IRI activity, which has many biotechnological applications.

Original languageEnglish
Pages (from-to)478-485
Number of pages8
JournalBiomaterials Science
Volume1
Issue number5
DOIs
Publication statusPublished - 2013

Keywords

  • RED-BLOOD-CELLS
  • ANTIFREEZE GLYCOPROTEINS
  • THERMAL HYSTERESIS
  • POLY(VINYL ALCOHOL)
  • PHYSICAL-PROPERTIES
  • AQUEOUS-SOLUTION
  • PROTEINS
  • CRYOPRESERVATION
  • TISSUES
  • ANALOGS

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