Identification and characterization of the UL24 gene product of herpes simplex virus type 2

Z Hong-Yan, T Murata, F Goshima, H Takakuwa, T Koshizuka, Y Yamauchi, Y Nishiyama

Research output: Contribution to journalArticle (Academic Journal)

19 Citations (Scopus)

Abstract

The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.

Original languageEnglish
Pages (from-to)321-7
Number of pages7
JournalVirus Genes
Volume22
Issue number3
Publication statusPublished - Jun 2001

Keywords

  • Animals
  • Base Sequence
  • COS Cells
  • Capsid
  • DNA Primers
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique, Indirect
  • Herpesvirus 2, Human
  • Immune Sera
  • Viral Proteins
  • Virion
  • Journal Article
  • Research Support, Non-U.S. Gov't

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  • Cite this

    Hong-Yan, Z., Murata, T., Goshima, F., Takakuwa, H., Koshizuka, T., Yamauchi, Y., & Nishiyama, Y. (2001). Identification and characterization of the UL24 gene product of herpes simplex virus type 2. Virus Genes, 22(3), 321-7.