Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda': A step towards rust proofing wheat cultivation

Gomti Grover; Achla Sharma; Ian Mackay; Puja Srivastava; Satinder Kaur; Jaspal Kaur; Amanda Burridge; Sacha Przewieslik Allen; Alison R Bentley; Parveen Chhuneja; N S Bains

doi:10.1371/journal.pone.0264027

Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda': A step towards rust proofing wheat cultivation

Gomti Grover, Achla Sharma, Ian Mackay, Puja Srivastava, Satinder Kaur, Jaspal Kaur, Amanda Burridge, Sacha Przewieslik Allen, Alison R Bentley, Parveen Chhuneja, N S Bains

School of Biological Sciences

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Abstract

All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar 'Acienda'. In order to dissect the genetic basis of the resistance, a backcross population was developed between 'Acienda' and the stripe rust susceptible Indian spring wheat cultivar 'HD 2967'. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from 'Acienda' has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.

Original language	English
Article number	e0264027
Pages (from-to)	e0264027
Journal	PLoS ONE
Volume	17
Issue number	2
Early online date	16 Feb 2022
DOIs	https://doi.org/10.1371/journal.pone.0264027
Publication status	E-pub ahead of print - 16 Feb 2022

Bibliographical note

Funding Information:
The first author acknowledges the fellowship provide during her Ph.D program by Department of Science & Technology, Govt. of India under 'INSPIRE fellowship scheme. All authors from PAU duly acknowledge Dr Kuldeep Singh, Director, National Bureau of Plant Genetic Resources for procuring the European winter wheat set from NIAB, UK and sharing it with Department of Plant Breeding and Genetics, PAU, Ludhiana.

Publisher Copyright:
© 2022 Grover et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Keywords

Basidiomycota/physiology
Chromosome Mapping
Chromosomes, Plant
Disease Resistance/genetics
Gene Expression Regulation, Plant
India
Inheritance Patterns
Plant Diseases/genetics
Plant Proteins/genetics
Polymorphism, Single Nucleotide
Quantitative Trait Loci
Seasons
Triticum/genetics

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Grover, G., Sharma, A., Mackay, I., Srivastava, P., Kaur, S., Kaur, J., Burridge, A., Allen, S. P., Bentley, A. R., Chhuneja, P., & Bains, N. S. (2022). Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda': A step towards rust proofing wheat cultivation. PLoS ONE, 17(2), e0264027. Article e0264027. Advance online publication. https://doi.org/10.1371/journal.pone.0264027

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abstract = "All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar 'Acienda'. In order to dissect the genetic basis of the resistance, a backcross population was developed between 'Acienda' and the stripe rust susceptible Indian spring wheat cultivar 'HD 2967'. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from 'Acienda' has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.",

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note = "Funding Information: The first author acknowledges the fellowship provide during her Ph.D program by Department of Science & Technology, Govt. of India under 'INSPIRE fellowship scheme. All authors from PAU duly acknowledge Dr Kuldeep Singh, Director, National Bureau of Plant Genetic Resources for procuring the European winter wheat set from NIAB, UK and sharing it with Department of Plant Breeding and Genetics, PAU, Ludhiana. Publisher Copyright: {\textcopyright} 2022 Grover et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

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Grover, G, Sharma, A, Mackay, I, Srivastava, P, Kaur, S, Kaur, J, Burridge, A , Allen, SP, Bentley, AR, Chhuneja, P & Bains, NS 2022, 'Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda': A step towards rust proofing wheat cultivation', PLoS ONE, vol. 17, no. 2, e0264027, pp. e0264027. https://doi.org/10.1371/journal.pone.0264027

TY - JOUR

T1 - Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda'

T2 - A step towards rust proofing wheat cultivation

AU - Grover, Gomti

AU - Sharma, Achla

AU - Mackay, Ian

AU - Srivastava, Puja

AU - Kaur, Satinder

AU - Kaur, Jaspal

AU - Burridge, Amanda

AU - Allen, Sacha Przewieslik

AU - Bentley, Alison R

AU - Chhuneja, Parveen

AU - Bains, N S

N1 - Funding Information: The first author acknowledges the fellowship provide during her Ph.D program by Department of Science & Technology, Govt. of India under 'INSPIRE fellowship scheme. All authors from PAU duly acknowledge Dr Kuldeep Singh, Director, National Bureau of Plant Genetic Resources for procuring the European winter wheat set from NIAB, UK and sharing it with Department of Plant Breeding and Genetics, PAU, Ludhiana. Publisher Copyright: © 2022 Grover et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2022/2/16

Y1 - 2022/2/16

N2 - All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar 'Acienda'. In order to dissect the genetic basis of the resistance, a backcross population was developed between 'Acienda' and the stripe rust susceptible Indian spring wheat cultivar 'HD 2967'. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from 'Acienda' has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.

AB - All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar 'Acienda'. In order to dissect the genetic basis of the resistance, a backcross population was developed between 'Acienda' and the stripe rust susceptible Indian spring wheat cultivar 'HD 2967'. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from 'Acienda' has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.

KW - Basidiomycota/physiology

KW - Chromosome Mapping

KW - Chromosomes, Plant

KW - Disease Resistance/genetics

KW - Gene Expression Regulation, Plant

KW - India

KW - Inheritance Patterns

KW - Plant Diseases/genetics

KW - Plant Proteins/genetics

KW - Polymorphism, Single Nucleotide

KW - Quantitative Trait Loci

KW - Seasons

KW - Triticum/genetics

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U2 - 10.1371/journal.pone.0264027

DO - 10.1371/journal.pone.0264027

M3 - Article (Academic Journal)

C2 - 35171951

SN - 1932-6203

VL - 17

SP - e0264027

JO - PLoS ONE

JF - PLoS ONE

IS - 2

M1 - e0264027

ER -

Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda': A step towards rust proofing wheat cultivation

Abstract

Bibliographical note

Keywords

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