Identification of a novel stripe rust resistance gene from the European winter wheat cultivar 'Acienda': A step towards rust proofing wheat cultivation

Gomti Grover, Achla Sharma, Ian Mackay, Puja Srivastava, Satinder Kaur, Jaspal Kaur, Amanda Burridge, Sacha Przewieslik Allen, Alison R Bentley, Parveen Chhuneja, N S Bains

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar 'Acienda'. In order to dissect the genetic basis of the resistance, a backcross population was developed between 'Acienda' and the stripe rust susceptible Indian spring wheat cultivar 'HD 2967'. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from 'Acienda' has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.

Original languageEnglish
Article numbere0264027
Pages (from-to)e0264027
JournalPLoS ONE
Volume17
Issue number2
Early online date16 Feb 2022
DOIs
Publication statusE-pub ahead of print - 16 Feb 2022

Bibliographical note

Funding Information:
The first author acknowledges the fellowship provide during her Ph.D program by Department of Science & Technology, Govt. of India under 'INSPIRE fellowship scheme. All authors from PAU duly acknowledge Dr Kuldeep Singh, Director, National Bureau of Plant Genetic Resources for procuring the European winter wheat set from NIAB, UK and sharing it with Department of Plant Breeding and Genetics, PAU, Ludhiana.

Publisher Copyright:
© 2022 Grover et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Keywords

  • Basidiomycota/physiology
  • Chromosome Mapping
  • Chromosomes, Plant
  • Disease Resistance/genetics
  • Gene Expression Regulation, Plant
  • India
  • Inheritance Patterns
  • Plant Diseases/genetics
  • Plant Proteins/genetics
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci
  • Seasons
  • Triticum/genetics

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