Identification of amino acids in the dengue virus type 2 envelope glycoprotein critical to virus infectivity

H Kroschewski, J-L Sagripanti, AD Davidson

Research output: Contribution to journalArticle (Academic Journal)peer-review

10 Citations (Scopus)

Abstract

The dengue virus envelope glycoprotein mediates virus attachment and entry and is the major viral antigen. The identification of ‘critical’ amino acids in the envelope glycoprotein that cannot be altered without loss of infectivity could have a major impact on the development of dengue virus vaccines and diagnostics. In this context, we determined whether six amino acids, previously predicted by computational analysis to play a critical role in the virus life cycle, were essential for virus viability. The effects of mutating the six ‘critical’ amino acids and a further seven ‘neutral’ amino acids were analysed by using a dengue virus type 2 infectious cDNA clone. Of the six critical amino acids, three (Asp-215, Pro-217 and His-244) were found to be essential for virus viability in mammalian and mosquito cells.
Translated title of the contributionIdentification of amino acids in the dengue virus type 2 envelope glycoprotein critical to virus infectivity
Original languageEnglish
Pages (from-to)2457 - 2461
Number of pages5
JournalJournal of General Virology
Volume90
DOIs
Publication statusPublished - Jul 2009

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