Angiostrongylus vasorum is an emerging parasite of dogs and related carnivores throughout western Europe and presents a biosecurity threat to many countries worldwide. Infections are difficult to diagnose due to a high variability of clinical signs and can be fatal if left untreated. Previous attempts to develop indirect ELISA as a diagnostic test for antibody presence in dogs have been limited by cross-reactive recognition of antibodies to other endemic nematodes. This study compared the immuno-dominant soluble somatic proteins for first stage, third stage and adult A. vasorum using Western blotting. 2D electrophoresis and mass spectrometry were further used to identify specific immuno-dominant proteins (n = 14) within the soluble fraction from whole adult worms. Proteins included an aspartyl protease inhibitor, representing a potential candidate for a recombinant-based ELISA and novel vaccine.
|Translated title of the contribution||Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry|
|Pages (from-to)||56 - 61|
|Number of pages||6|
|Journal||Molecular & Biochemical Parasitology|
|Publication status||Published - Nov 2011|
Bibliographical noteOther: First published on-line 4th August 2011
Jefferies, R., Morgan, ER., Shaw, S., & Heesom, K. (2011). Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry. Molecular & Biochemical Parasitology, 180 (1), 56 - 61. https://doi.org/10.1016/j.molbiopara.2011.07.009