Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry

R Jefferies, ER Morgan, S Shaw, K Heesom

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)

Abstract

Angiostrongylus vasorum is an emerging parasite of dogs and related carnivores throughout western Europe and presents a biosecurity threat to many countries worldwide. Infections are difficult to diagnose due to a high variability of clinical signs and can be fatal if left untreated. Previous attempts to develop indirect ELISA as a diagnostic test for antibody presence in dogs have been limited by cross-reactive recognition of antibodies to other endemic nematodes. This study compared the immuno-dominant soluble somatic proteins for first stage, third stage and adult A. vasorum using Western blotting. 2D electrophoresis and mass spectrometry were further used to identify specific immuno-dominant proteins (n = 14) within the soluble fraction from whole adult worms. Proteins included an aspartyl protease inhibitor, representing a potential candidate for a recombinant-based ELISA and novel vaccine.
Translated title of the contributionIdentification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry
Original languageEnglish
Pages (from-to)56 - 61
Number of pages6
JournalMolecular & Biochemical Parasitology
Volume180 (1)
DOIs
Publication statusPublished - Nov 2011

Bibliographical note

Other: First published on-line 4th August 2011

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