Identification of methyl halide-utilizing genes in the methyl bromide-utilizing bacterial strain IMB-1 suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria

C A Woodall, K L Warner, R S Oremland, J C Murrell, I R McDonald

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyltransferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.

Original languageEnglish
Pages (from-to)1959-63
Number of pages5
JournalApplied and Environmental Microbiology
Volume67
Issue number4
DOIs
Publication statusPublished - 10 Apr 2001

Keywords

  • Alphaproteobacteria/genetics
  • Amino Acid Sequence
  • Bacterial Proteins
  • Culture Media
  • Genes, Bacterial
  • Gram-Negative Bacteria/genetics
  • Hydrocarbons, Brominated/metabolism
  • Methyltransferases/chemistry
  • Molecular Sequence Data
  • Sequence Analysis, DNA

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