Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly

L Peacock, VR Ferris, R Sharma, J Sunter, M Bailey, M Carrington, WC Gibson

Research output: Contribution to journalArticle (Academic Journal)peer-review

88 Citations (Scopus)

Abstract

Elucidating the mechanism of genetic exchange is fundamental for understanding how genes for such traits as virulence, disease phenotype, and drug resistance are transferred between pathogen strains. Genetic exchange occurs in the parasitic protists Trypanosoma brucei, T. cruzi, and Leishmania major, but the precise cellular mechanisms are unknown, because the process has not been observed directly. Here we exploit the identification of homologs of meiotic genes in the T. brucei genome and demonstrate that three functionally distinct, meiosis-specific proteins are expressed in the nucleus of a single specific cell type, defining a previously undescribed developmental stage occurring within the tsetse fly salivary gland. Expression occurs in clonal and mixed infections, indicating that the meiotic program is an intrinsic but hitherto cryptic part of the developmental cycle of trypanosomes. In experimental crosses, expression of meiosis-specific proteins usually occurred before cell fusion. This is evidence of conventional meiotic division in an excavate protist, and the functional conservation of the meiotic machinery in these divergent organisms underlines the ubiquity and basal evolution of meiosis in eukaryotes.
Translated title of the contributionIdentification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly
Original languageEnglish
Pages (from-to)3671 - 3676
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number9
DOIs
Publication statusPublished - Mar 2011

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