Skip to content

Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson’s Disease Research

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Original languageEnglish
Title of host publicationAutophagy
Publisher or commissioning bodyHumana Press
Pages257-280
Number of pages23
DOIs
DateAccepted/In press - 29 Sep 2018
DatePublished (current) - 5 Jan 2019

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Electronic)1064-3745

Abstract

To appreciate the positive or negative impact of autophagy during the initiation and progression of human diseases, the isolation or de novo generation of appropriate cell types is required to support focused in vitro assays. In human neurodegenerative diseases such as Parkinson’s disease (PD), specific subsets of acutely sensitive neurons become susceptible to stress-associated operational decline and eventual cell death, emphasizing the need for functional studies in those vulnerable groups of neurons. In PD, a class of dopaminergic neurons in the ventral midbrain (mDANs) is affected. To study these, human-induced pluripotent stem cells (hiPSCs) have emerged as a valuable tool, as they enable the establishment and study of mDAN biology in vitro. In this chapter, we describe a stepwise protocol for the generation of mDANs from hiPSCs using a monolayer culture system. We then outline how imaging-based autophagy assessment methodologies can be applied to these neurons, thereby providing a detailed account of the application of imaging-based autophagy assays to human iPSC-derived mDANs

    Research areas

  • Autophagy, Parkinson’s disease, Immunofluorescence, Cell culture, Dopaminergic neurons, Stem cells, hiPSC

Download statistics

No data available

Documents

Documents

  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Springer Nature at https://www.springer.com/la/book/9781493988723 . Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 4.03 MB, PDF document

DOI

View research connections

Related faculties, schools or groups