Imaging dynamic biological processes with ESEM – A proof of principle study on plant tissue

JE McGregor, AM Donald

Research output: Chapter in Book/Report/Conference proceedingConference Contribution (Conference Proceeding)

Abstract

The Environmental Scanning Electron Microscope (ESEM) differs from a conventional SEM in that a differential pumping system maintains a pressure of gas (typically H2O) in the specimen chamber whilst the gun remains at high vacuum [1]. Ionizing collisions between electrons and these gas molecules create positive ions which drift down onto the sample providing a mechanism for neutralising specimen charge. It is therefore possible to image insulating samples without the need for a metallic coating. The presence of water vapour in the chamber also means that a high relative humidity can be maintained and therefore samples can be imaged in a hydrated state without the need for dehydration and fixation.
Translated title of the contributionImaging dynamic biological processes with ESEM – A proof of principle study on plant tissue
Original languageEnglish
Title of host publicationMC2009 Graz
EditorsM.A. Pabst, G. Zellnig
PublisherTU Graz
Pages133 - 134
Volume2, Life Sciences
DOIs
Publication statusPublished - 2009

Bibliographical note

Conference Proceedings/Title of Journal: MC2009

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