Imaging rRNA Methylation in Bacteria by MR-FISH

Martin R Challand

Research output: Chapter in Book/Report/Conference proceedingChapter in a book


Methylation of RNA is normally monitored in purified cell lysates using next-generation sequencing, gel electrophoresis, or mass spectrometry as readouts. These bulk methods require the RNA from ~104 to 107 cells to be pooled to generate sufficient material for analysis. Here we describe a method—methylation-sensitive RNA in situ hybridization (MR-FISH)—that assays rRNA methylation in bacteria on a cell-by-cell basis, using methylation-sensitive hybridization probes and fluorescence microscopy. We outline step-by-step protocols for designing probes, in situ hybridization, and analysis of data using freely available code
Original languageEnglish
Title of host publicationImaging Gene Expression
PublisherHumana Press
Number of pages18
ISBN (Electronic)978-1-4939-9674-2
ISBN (Print)978-1-4939-9673-5
Publication statusPublished - 13 Aug 2019

Publication series

NameMethods in Molecular Biology


Dive into the research topics of 'Imaging rRNA Methylation in Bacteria by MR-FISH'. Together they form a unique fingerprint.

Cite this