Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

Imke Sittel, Carmen Galan

Research output: Contribution to journalArticle (Academic Journal)peer-review

4 Citations (Scopus)
260 Downloads (Pure)

Abstract

Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, ITag-LacNAc, ITag-Lewisx and ITag-Lewisa, in a matter of days from human breast milk without having to isolate specific enzymes.
Original languageEnglish
Pages (from-to)3575-3579
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume15
Issue number17
Early online date6 Apr 2017
DOIs
Publication statusPublished - 7 May 2017

Keywords

  • Directed biosynthesis
  • Glycosylation
  • Human milk
  • Glycosyltransferases
  • Imidazolium labels

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