Projects per year
Abstract
Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, ITag-LacNAc, ITag-Lewisx and ITag-Lewisa, in a matter of days from human breast milk without having to isolate specific enzymes.
Original language | English |
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Pages (from-to) | 3575-3579 |
Number of pages | 5 |
Journal | Organic and Biomolecular Chemistry |
Volume | 15 |
Issue number | 17 |
Early online date | 6 Apr 2017 |
DOIs | |
Publication status | Published - 7 May 2017 |
Keywords
- Directed biosynthesis
- Glycosylation
- Human milk
- Glycosyltransferases
- Imidazolium labels
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Dive into the research topics of 'Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk'. Together they form a unique fingerprint.Projects
- 2 Finished
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3-month Core Capability for Chemistry Research
Crosby, J. (Principal Investigator)
1/01/13 → 1/04/13
Project: Research
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Reworking of Novel tools for Glycoscience
Galan, M. C. (Principal Investigator)
31/03/12 → 30/08/17
Project: Research