Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum

Yao-Yao Zhang; Mattia Ghirardello; Ting Wang; Ai-Min Lu; Li Liu; Josef Voglmeir; M Carmen Galan

doi:10.1039/d1cc02100a

Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum

Yao-Yao Zhang, Mattia Ghirardello, Ting Wang, Ai-Min Lu, Li Liu, Josef Voglmeir, M Carmen Galan

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Abstract

A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.

Original language	English
Pages (from-to)	7003-7006
Number of pages	4
Journal	Chemical communications (Cambridge, England)
Volume	57
Issue number	57
Early online date	17 Jun 2021
DOIs	https://doi.org/10.1039/d1cc02100a
Publication status	Published - 21 Jul 2021

Bibliographical note

Funding Information:
This study was funded by the National Natural Science Foundation of China (NSFC) 31471703, 31671854, 31871793 and 31871754 (to JV and LL), and ERC-COG: 648239 (MCG).

Publisher Copyright:
© The Royal Society of Chemistry 2021.

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title = "Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum",

abstract = "A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.",

author = "Yao-Yao Zhang and Mattia Ghirardello and Ting Wang and Ai-Min Lu and Li Liu and Josef Voglmeir and Galan, {M Carmen}",

note = "Funding Information: This study was funded by the National Natural Science Foundation of China (NSFC) 31471703, 31671854, 31871793 and 31871754 (to JV and LL), and ERC-COG: 648239 (MCG). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

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T1 - Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum

AU - Zhang, Yao-Yao

AU - Ghirardello, Mattia

AU - Wang, Ting

AU - Lu, Ai-Min

AU - Liu, Li

AU - Voglmeir, Josef

AU - Galan, M Carmen

N1 - Funding Information: This study was funded by the National Natural Science Foundation of China (NSFC) 31471703, 31671854, 31871793 and 31871754 (to JV and LL), and ERC-COG: 648239 (MCG). Publisher Copyright: © The Royal Society of Chemistry 2021.

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Y1 - 2021/7/21

N2 - A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.

AB - A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.

U2 - 10.1039/d1cc02100a

DO - 10.1039/d1cc02100a

M3 - Article (Academic Journal)

C2 - 34159978

SN - 1359-7345

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JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

IS - 57

ER -

Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum

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