Imidazolium-tagged glycan probes for non-covalent labeling of live cells

David Benito-Alifonso; Shirley Tremell; Joanna C Sadler; Monica Berry; M Carmen Galan

doi:10.1039/c5cc10040b

Imidazolium-tagged glycan probes for non-covalent labeling of live cells

David Benito-Alifonso, Shirley Tremell, Joanna C Sadler, Monica Berry, M Carmen Galan

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

Original language	English
Pages (from-to)	4906-4909
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	27
DOIs	https://doi.org/10.1039/c5cc10040b
Publication status	Published - 7 Apr 2016

Research Groups and Themes

Bristol BioDesign Institute
Organic & Biological

Keywords

SYNTHETIC BIOLOGY
synthetic biology

Access to Document

10.1039/c5cc10040b

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This is the final published version of the article (version of record). It first appeared online via Royal Society of Chemistry at http://dx.doi.org/10.1039/C5CC10040B. Please refer to any applicable terms of use of the publisher.
Final published version, 2.08 MBLicence: CC BY

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3-month Core Capability for Chemistry Research
Crosby, J. (Principal Investigator)
1/01/13 → 1/04/13
Project: Research
Reworking of Novel tools for Glycoscience
Galan, M. C. (Principal Investigator)
31/03/12 → 30/08/17
Project: Research

Wolfson Bioimaging Facility
Jepson, M. (Manager)
Facility/equipment: Facility

Cite this

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title = "Imidazolium-tagged glycan probes for non-covalent labeling of live cells",

abstract = "Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.",

keywords = "SYNTHETIC BIOLOGY, synthetic biology",

author = "David Benito-Alifonso and Shirley Tremell and Sadler, \{Joanna C\} and Monica Berry and Galan, \{M Carmen\}",

year = "2016",

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doi = "10.1039/c5cc10040b",

language = "English",

volume = "52",

pages = "4906--4909",

journal = "Chemical Communications",

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T1 - Imidazolium-tagged glycan probes for non-covalent labeling of live cells

AU - Benito-Alifonso, David

AU - Tremell, Shirley

AU - Sadler, Joanna C

AU - Berry, Monica

AU - Galan, M Carmen

PY - 2016/4/7

Y1 - 2016/4/7

N2 - Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

AB - Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

KW - SYNTHETIC BIOLOGY

KW - synthetic biology

U2 - 10.1039/c5cc10040b

DO - 10.1039/c5cc10040b

M3 - Article (Academic Journal)

C2 - 26974358

SN - 1359-7345

VL - 52

SP - 4906

EP - 4909

JO - Chemical Communications

JF - Chemical Communications

IS - 27

ER -

Imidazolium-tagged glycan probes for non-covalent labeling of live cells

Abstract

Research Groups and Themes

Keywords

Access to Document

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Projects

3-month Core Capability for Chemistry Research

Reworking of Novel tools for Glycoscience

Equipment

Wolfson Bioimaging Facility

Cite this