Immune development in jejunal mucosa after colonization with selected commensal gut bacteria: A study in germ-free pigs

K Haverson, Z Rehakova, J Sinkora, L Sver, M Bailey

Research output: Contribution to journalArticle (Academic Journal)

38 Citations (Scopus)

Abstract

The immunological structure of the porcine jejunal lamina propria in germ-free piglets was compared with that of their counterparts associated with two strains of commensal Escherichia coli, A0 34/86 serotype O83:K24:H31 and the O86 E. coli strain, up to 20 days post-colonization. In the antigen-presenting compartment, both dendritic cells (DC) and cells expressing CD163, probably macrophages were investigated. In addition we also assessed the number of CD2+/CD3+ (T) cells. In contrast to some previous reports, we show a total lack of both DC and T cells for germ-free animals in the diffuse lymphoid tissue of villi and crypts of the jejunum. Association with either strain of commensal E. coli had a profound effect on the immune structure and resulted in extensive recruitment of DC to the lamina propria and of T cells to epithelium and lamina propria. The data suggest that the earliest immigrant cells were monocytes, which soon acquired the phenotype of mucosal DC. T cells migrated in at a slightly slower rate. Nevertheless, the response could be extremely rapid: within 3 days of colonization with O83, the magnitude of this response was comparable to that observed 20 days post-colonization.
Translated title of the contributionImmune development in jejunal mucosa after colonization with selected commensal gut bacteria: A study in germ-free pigs
Original languageEnglish
Pages (from-to)243 - 253
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume119 (3-4)
DOIs
Publication statusPublished - Oct 2007

Bibliographical note

Publisher: Elsevier

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