Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

Valerie A Ferro, Rosaria Costa, K Christine Carter, Michael J A Harvey, Mary M Waterston, Alexander B Mullen, Christian Matschke, Jamie F S Mann, Angela Colston, William H Stimson

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by examining specific antibody levels, antibody subclasses, endocrine ablation and gonadal atrophy. The production of IgG2b antibody provided the best correlation for castration. PLGA was considered the most effective adjuvant as it produced a consistent anti-fertility response in all the treated animals.

Original languageEnglish
Pages (from-to)1024-31
Number of pages8
JournalVaccine
Volume22
Issue number8
DOIs
Publication statusPublished - 25 Feb 2004

Keywords

  • Adjuvants, Immunologic/pharmacology
  • Animals
  • Antibody Formation
  • Gonadotropin-Releasing Hormone/chemistry
  • Gonadotropins/blood
  • Immunization
  • Immunoglobulin G/blood
  • Immunoglobulin M/blood
  • Male
  • Oligopeptides/chemistry
  • Organ Size/drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Testis/drug effects
  • Testosterone/blood
  • Tetanus Toxoid/chemistry
  • Vaccines, Contraceptive/chemistry

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