TY - JOUR
T1 - Immunity in rainbow trout, Oncorhynchus mykiss, against the monogenean Discocotyle sagittata following primary infection
AU - Rubio-Godoy, Miguel
AU - Tinsley, Richard C.
PY - 2004/3/1
Y1 - 2004/3/1
N2 - Rainbow trout (Oncorhynchus mykiss) were experimentally infected by continuous or single exposure with the monogenean Discocotyle sagittata. To determine whether immunity follows primary infection, fish were exposed to a secondary challenge by one of two modes: (1) primary infections were cleared with praziquantel (PZQ) and hosts re-infected with 100 oncomiracidia; (2) parasites were allowed to reach maturity and hosts super-infected with 100 oncomiracidia. Fish challenged after initial continuous exposure developed significant partial resistance to re-infection, carrying burdens 35% lower than controls. PZQ treatment controls demonstrated that the drug did not account for the protection observed. Single exposure did not significantly modify the outcome of secondary challenges. Super-infection experiments suggested that no concomitant immunity develops. No correlation was found between initial burdens and the outcome of secondary challenges in the same individual. Significantly elevated anti-D. sagittata antibodies were detected in infected fish, but there was no correlation between immunoglobulin levels and parasite burdens.
AB - Rainbow trout (Oncorhynchus mykiss) were experimentally infected by continuous or single exposure with the monogenean Discocotyle sagittata. To determine whether immunity follows primary infection, fish were exposed to a secondary challenge by one of two modes: (1) primary infections were cleared with praziquantel (PZQ) and hosts re-infected with 100 oncomiracidia; (2) parasites were allowed to reach maturity and hosts super-infected with 100 oncomiracidia. Fish challenged after initial continuous exposure developed significant partial resistance to re-infection, carrying burdens 35% lower than controls. PZQ treatment controls demonstrated that the drug did not account for the protection observed. Single exposure did not significantly modify the outcome of secondary challenges. Super-infection experiments suggested that no concomitant immunity develops. No correlation was found between initial burdens and the outcome of secondary challenges in the same individual. Significantly elevated anti-D. sagittata antibodies were detected in infected fish, but there was no correlation between immunoglobulin levels and parasite burdens.
UR - http://www.scopus.com/inward/record.url?scp=1842688998&partnerID=8YFLogxK
U2 - 10.1007/s00436-003-1056-6
DO - 10.1007/s00436-003-1056-6
M3 - Article (Academic Journal)
C2 - 14740206
AN - SCOPUS:1842688998
VL - 92
SP - 367
EP - 374
JO - Parasitology Research
JF - Parasitology Research
SN - 0932-0113
IS - 5
ER -