Immunocytochemistry and immunohistochemistry

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

In immunocytochemistry and immunohistochemistry techniques, an antibody is
used to specifically label a cellular antigen (e.g. a protein). These localization
approaches fundamentally rely on the high specificity, affinity and sensitivity of
antibody-antigen interactions. Antibodies are visualized either directly or indirectly
(usually via a secondary antibody), with a stain that is easily detectable under a light or
electron microscope. An ideal stain is stable, will reveal accurately the distribution of
the antigen and is suitable for both low- and high-resolution localization studies. By
convention, the immunochemical detection of an antigen in tissues is known as
immunohistochemistry, and detection in cells is termed immunocytochemistry.
Original languageEnglish
Title of host publicationEssential Guide to Reading Biomedical Papers
Subtitle of host publicationRecognising and Interpreting Best Practice
EditorsPD Langton
PublisherWiley-Blackwell
Pages117-127
Number of pages11
ISBN (Print)978-1-1199-5996-0
Publication statusPublished - 2013

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