Abstract
In immunocytochemistry and immunohistochemistry techniques, an antibody is
used to specifically label a cellular antigen (e.g. a protein). These localization
approaches fundamentally rely on the high specificity, affinity and sensitivity of
antibody-antigen interactions. Antibodies are visualized either directly or indirectly
(usually via a secondary antibody), with a stain that is easily detectable under a light or
electron microscope. An ideal stain is stable, will reveal accurately the distribution of
the antigen and is suitable for both low- and high-resolution localization studies. By
convention, the immunochemical detection of an antigen in tissues is known as
immunohistochemistry, and detection in cells is termed immunocytochemistry.
used to specifically label a cellular antigen (e.g. a protein). These localization
approaches fundamentally rely on the high specificity, affinity and sensitivity of
antibody-antigen interactions. Antibodies are visualized either directly or indirectly
(usually via a secondary antibody), with a stain that is easily detectable under a light or
electron microscope. An ideal stain is stable, will reveal accurately the distribution of
the antigen and is suitable for both low- and high-resolution localization studies. By
convention, the immunochemical detection of an antigen in tissues is known as
immunohistochemistry, and detection in cells is termed immunocytochemistry.
| Original language | English |
|---|---|
| Title of host publication | Essential Guide to Reading Biomedical Papers |
| Subtitle of host publication | Recognising and Interpreting Best Practice |
| Editors | PD Langton |
| Publisher | Wiley-Blackwell |
| Pages | 117-127 |
| Number of pages | 11 |
| ISBN (Print) | 978-1-1199-5996-0 |
| Publication status | Published - 2013 |