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Impact by pancreatic stellate cells on epithelial-mesenchymal transition and pancreatic cancer cell invasion: adding a third dimension in vitro

Research output: Contribution to journalArticle

  • Emelie Karnevi
  • Ann H Rosendahl
  • Katarzyna Said Hilmersson
  • Moin A Saleem
  • Roland Andersson
Original languageEnglish
Pages (from-to)206-215
Number of pages10
JournalExperimental Cell Research
Volume346
Issue number2
Early online date19 Jul 2016
DOIs
DateAccepted/In press - 17 Jul 2016
DateE-pub ahead of print - 19 Jul 2016
DatePublished (current) - 15 Aug 2016

Abstract

Pancreatic cancer is associated with a highly abundant stroma and low-grade inflammation. In the local tumour microenvironment, elevated glucose levels, the presence of tumour-associated stellate cells and macrophages are hypothesized to promote the tumour progression and invasion. The present study investigated the influence by the microenvironment on pancreatic cancer cell invasion in vitro. After co-culture with tumour-associated pancreatic stellate cells (TPSCs), pancreatic cancer cells displayed up to 8-fold reduction in levels of epithelial-mesenchymal transition (EMT) markers E-cadherin and ZO-1, while β-catenin and vimentin levels were increased. A 3D organotypic model showed that TPSCs stimulated pancreatic cancer cell invasion, both as single cell (PANC-1) and cohort (MIAPaCa-2) invasion. The combined presence of TPSCs and M2-like macrophages induced invasion of the non-invasive BxPC-3 cells. High glucose conditions further enhanced changes in EMT markers as well as the cancer cell invasion. In summary, co-culture with TPSCs induced molecular changes associated with EMT in pancreatic cancer cells, regardless of differentiation status, and the organotypic model demonstrated the influence of microenvironmental factors, such as glucose, stellate cells and macrophages, on pancreatic cancer cell invasion.

    Research areas

  • Pancreatic cancer, Pancreatic stellate cells, Macrophages, Epithelial-mesenchymal transition, Invasion, 3D organotypic model

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  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Elsevier at http://www.sciencedirect.com/science/article/pii/S0014482716302063. Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 510 KB, PDF document

    Licence: CC BY-NC-ND

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