Impaired growth plate function in bmp-6 null mice

Mark J Perry, Kathleen E McDougall, Shu-Chen Hou, Jonathan H Tobias

Research output: Contribution to journalArticle (Academic Journal)peer-review

26 Citations (Scopus)

Abstract

Bone morphogenetic protein 6 (BMP-6) is expressed by different skeletal cells including osteoblasts and growth plate chondrocytes, suggesting roles in bone formation and growth regulation. To address these possibilities, we examined whether cancellous and cortical bone parameters, or indices of growth plate function, are altered in bmp-6 null mice as assessed under basal conditions, and following stimulation of bone formation and suppression of growth by estrogen treatment. Ten-week-old female littermate bmp-6 null and wild-type (WT) mice were administered vehicle or E(2) 4, 40, 400 or 4,000 microg/kg/day by daily sc injection for 28 days (6-8 per group). Tibias were removed, and detailed histomorphometric analysis of the proximal metaphysis and growth plates, and tibial diaphysis were performed on longitudinal and transverse sections respectively. Long bone area as measured by DXA was reduced in vehicle-treated bmp-6 null mice compared with WT littermate controls. In addition, vehicle-treated bmp-6 null mice had a reduced cross-sectional area at the tibial mid-diaphysis as assessed by histomorphometry, whereas cancellous bone indices were unaffected. Histomorphometry of the proximal tibial metaphysis demonstrated a defect in bone formation immediately adjacent to the growth plate in bmp-6 null mice compared to WT mice following E(2) treatment. E(2) administration was also associated with a dose-responsive decrease in longitudinal growth rate, and proliferative and hypertrophic zone parameters of the growth plate (p
Translated title of the contributionImpaired growth plate function in bmp-6 null mice
Original languageEnglish
Pages (from-to)216 - 225
Number of pages10
JournalBone
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 2008

Bibliographical note

Publisher: Elsevier

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