Impaired OMA1 dependent OPA1 cleavage and reduced DRP1 fission activity combine to prevent mitophagy in OXPHOS dependent cells

Tom MacVicar, Jon D Lane

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Mitochondrial dynamics play crucial roles in mitophagy-based mitochondrial quality control, but how these pathways are regulated to meet cellular energy demands remains obscure. Using non-transformed human RPE1 cells, we report that upregulation of mitochondrial OXPHOS alters mitochondrial dynamics to inhibit Parkin-mediated mitophagy. Despite basal mitophagy rates remaining stable upon switch to OXPHOS-dependency, mitochondria resist fragmentation when RPE1 cells are treated with the protonophore, CCCP. Mechanistically, this is because cleavage of the inner membrane fusion factor L-OPA1 is prevented due to the failure to activate the inner membrane protease OMA1 in mitochondria with collapsed membrane potential. In parallel, OXPHOS-active mitochondria are protected from damage-induced fission, through impaired recruitment and activation of mitochondrial DRP1. Using OMA1-deficient MEFs, we show that the preservation of a stable pool of L-OPA1 at the inner mitochondrial membrane is sufficient to delay mitophagy, even in the presence of Parkin. The capacity of OXPHOS-dependent cells to maintain significant mitochondrial content in the face of acute damage has important implications for mitochondrial quality control in vivo.
Original languageEnglish
Article number15
Pages (from-to)2313-2325
JournalJournal of Cell Science
DOIs
Publication statusPublished - 2014

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