Improving the success rate of protein crystallization by random microseed matrix screening

Marisa Till, Alice Robson, Matt Byrne, Asha Nair, Stefan Kolek, Patrick Shaw Stewart, Paul Race

Research output: Contribution to journalArticle (Academic Journal)

Abstract

Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.
Original languageEnglish
JournalJournal of Visualized Experiments
Volume78
Publication statusPublished - 31 Aug 2013

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