Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.
|Journal||Journal of Visualized Experiments|
|Publication status||Published - 31 Aug 2013|
Till, M., Robson, A., Byrne, M., Nair, A., Kolek, S., Shaw Stewart, P., & Race, P. (2013). Improving the success rate of protein crystallization by random microseed matrix screening. Journal of Visualized Experiments, 78.