Abstract
Maleidrides are a family of structurally related fungal natural products, many of which possess diverse, potent bioactivities. Previous identification of several maleidride biosynthetic gene clusters, and subsequent experimental work, has determined the ‘core’ set of genes required to construct the characteristic medium-sized alicyclic ring with maleic anhydride moieties. Through genome mining, this work has used these core genes to discover ten entirely novel putative maleidride biosynthetic gene clusters, amongst both publicly available genomes, and encoded within the genome of the previously un-sequenced epiheveadride producer Wicklowia aquatica CBS 125634. We have undertaken phylogenetic analyses and comparative bioinformatics on all known and putative maleidride biosynthetic gene clusters to gain further insights regarding these unique biosynthetic pathways.
| Original language | English |
|---|---|
| Article number | 2 |
| Number of pages | 21 |
| Journal | Fungal Biology and Biotechnology |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 17 Feb 2022 |
Bibliographical note
Funding Information:We would like to thank the MRC for funding KW and KMJDMS (MR/N029909/1). Work conducted with W. aquatica, including genome sequencing and bioinformatics, was funded by the BBSRC (BB/K002341/1) and Syngenta.
Funding Information:
We would like to thank Professor Russell J. Cox for the inspiration for this work. We would like to thank Dr Jason L. Vincent, Dr Nicholas P. Mulholland and Dr Lauren Ray from Syngenta for their support and funding for this project. Genome sequencing and the production of the assembled draft genome of Wicklowia aquatica was carried out at the DNA sequencing facility in the Biochemistry Department of Cambridge University, with special thanks to Dr Markiyan Samborskyy for assembling the draft W. aquatica genome. We would like to thank Dr Claudio Greco for useful discussions on this work.
Publisher Copyright:
© 2022, The Author(s).