In situ cryo-electron tomography reveals filamentous actin within the microtubule lumen

Research output: Contribution to journalArticle (Academic Journal)

26 Downloads (Pure)

Abstract

Microtubules and filamentous (F-) actin engage in complex interactions to drive many cellular processes from subcellular organization to cell division and migration. This is thought to be largely controlled by proteins that interface between the two structurally distinct cytoskeletal components. Here, we use cryo-electron tomography to demonstrate that the microtubule lumen can be occupied by extended segments of F-actin in small molecule–induced, microtubule-based, cellular projections. We uncover an unexpected versatility in cytoskeletal form that may prompt a significant development of our current models of cellular architecture and offer a new experimental approach for the in situ study of microtubule structure and contents.
Original languageEnglish
Article numbere201911154
Number of pages14
JournalJournal of Cell Biology
Volume219
Issue number9
DOIs
Publication statusPublished - 1 Jun 2020

Fingerprint Dive into the research topics of 'In situ cryo-electron tomography reveals filamentous actin within the microtubule lumen'. Together they form a unique fingerprint.

  • Cite this