In Situ Visualization of Block Copolymer Self-Assembly in Organic Media by Super-Resolution Fluorescence Microscopy

Charlotte Boott, Romain Laine, Pierre Mahou, John Finnegan, Erin Leitao, Stephen Webb, Clemens Kaminski, Ian Manners

Research output: Contribution to journalArticle (Academic Journal)peer-review

30 Citations (Scopus)
299 Downloads (Pure)

Abstract

Analytical methods that enable visualization of nanomaterials derived from solution self-assembly processes in organic solvents are highly desirable. Here, we demonstrate the use of stimulated emission depletion microscopy (STED) and single molecule localization microscopy (SMLM) to map living crystallization-driven block copolymer (BCP) self-assembly in organic media at the sub-diffraction scale. Four different dyes were successfully used for single-colour super-resolution imaging of the BCP nanostructures allowing micelle length distributions to be determined in situ. Dual-colour SMLM imaging was used to measure and compare the rate of addition of red fluorescent BCP to the termini of green fluorescent seed micelles to generate block comicelles. Although well-established for aqueous systems, the results highlight the potential of super-resolution microscopy techniques for the interrogation of self-assembly processes in organic media.
Original languageEnglish
Pages (from-to)18539–18542
Number of pages4
JournalChemistry - A European Journal
Volume21
Issue number51
Early online date10 Nov 2015
DOIs
Publication statusPublished - 14 Dec 2015

Structured keywords

  • BCS and TECS CDTs

Keywords

  • block copolymer
  • superresolution fluorescence microscopy
  • living crystallization-driven self-assembly
  • micelles
  • self-assembly

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