During angiogenesis, endothelial cells invade into the stromal matrix; a complex, structuredarray of extracellular matrix proteins. This three dimensional, deformable substrate alsocontains a mixture of angiogenic factors as well as embedded stromal cells. Interactionsbetween endothelial cells and the stromal tissue make complex and important contributions tothe process of angiogenesis; however, the composition of the stromal matrix is hard toreplicate in vitro. The co-culture angiogenesis assay is a long-term assay that uses fibroblaststo secrete and condition a stromal matrix that more closely mimics tissue than a simplecollagen gel. Like all in vitro assays of angiogenesis, it has both strengths and weaknesses. Here we give protocols for the two of the most useful applications of the assay: screening forregulators of angiogenesis and high resolution imaging.
|Name||Methods in Molecular Biology|
- Endothelial cells