In Vitro Coculture Assays of Angiogenesis

Mark D Richards, Harry Mellor

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

During angiogenesis, endothelial cells invade into the stromal matrix; a complex, structuredarray of extracellular matrix proteins. This three dimensional, deformable substrate alsocontains a mixture of angiogenic factors as well as embedded stromal cells. Interactionsbetween endothelial cells and the stromal tissue make complex and important contributions tothe process of angiogenesis; however, the composition of the stromal matrix is hard toreplicate in vitro. The co-culture angiogenesis assay is a long-term assay that uses fibroblaststo secrete and condition a stromal matrix that more closely mimics tissue than a simplecollagen gel. Like all in vitro assays of angiogenesis, it has both strengths and weaknesses. Here we give protocols for the two of the most useful applications of the assay: screening forregulators of angiogenesis and high resolution imaging.
Original languageEnglish
Title of host publicationAngiogenesis Protocols
EditorsStewart G Martin, Peter W Hewett
PublisherSpringer
Pages159-166
Number of pages8
Volume2206
ISBN (Electronic)9781493936281
ISBN (Print)9781493936267
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1430
ISSN (Print)1064-3745

Keywords

  • Endothelial cells
  • angiogenesis
  • co-culture
  • imaging
  • fibroblasts
  • VEGF

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