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In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium

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In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium. / Nishiguchi, Akihiro; Gilmore, Catherine; Sood, Aman; Matsusaki, Michiya; Collett, Gavin; Tannetta, Dionne; Sargent, Ian L.; McGarvey, Jennifer; Halemani, Nagaraj D.; Hanley, Jon; Day, Fiona; Grant, Simon; Murdoch-Davis, Catherine; Kemp, Helena; Verkade, Paul; Aplin, John D.; Akashi, Mitsuru; Case, C. Patrick.

In: Biomaterials, Vol. 192, 01.02.2019, p. 140-148.

Research output: Contribution to journalArticle

Harvard

Nishiguchi, A, Gilmore, C, Sood, A, Matsusaki, M, Collett, G, Tannetta, D, Sargent, IL, McGarvey, J, Halemani, ND, Hanley, J, Day, F, Grant, S, Murdoch-Davis, C, Kemp, H, Verkade, P, Aplin, JD, Akashi, M & Case, CP 2019, 'In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium', Biomaterials, vol. 192, pp. 140-148. https://doi.org/10.1016/j.biomaterials.2018.08.025

APA

Nishiguchi, A., Gilmore, C., Sood, A., Matsusaki, M., Collett, G., Tannetta, D., ... Case, C. P. (2019). In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium. Biomaterials, 192, 140-148. https://doi.org/10.1016/j.biomaterials.2018.08.025

Vancouver

Nishiguchi A, Gilmore C, Sood A, Matsusaki M, Collett G, Tannetta D et al. In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium. Biomaterials. 2019 Feb 1;192:140-148. https://doi.org/10.1016/j.biomaterials.2018.08.025

Author

Nishiguchi, Akihiro ; Gilmore, Catherine ; Sood, Aman ; Matsusaki, Michiya ; Collett, Gavin ; Tannetta, Dionne ; Sargent, Ian L. ; McGarvey, Jennifer ; Halemani, Nagaraj D. ; Hanley, Jon ; Day, Fiona ; Grant, Simon ; Murdoch-Davis, Catherine ; Kemp, Helena ; Verkade, Paul ; Aplin, John D. ; Akashi, Mitsuru ; Case, C. Patrick. / In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium. In: Biomaterials. 2019 ; Vol. 192. pp. 140-148.

Bibtex

@article{d1257c9ad8264ea8bd8b2d249276240a,
title = "In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium",
abstract = "Fetal development may be compromised by adverse events at the placental interface between mother and fetus. However, it is still unclear how the communication between mother and fetus occurs through the placenta. In vitro - models of the human placental barrier, which could help our understanding and which recreate three-dimensional (3D) structures with biological functionalities and vasculatures, have not been reported yet. Here we present a 3D-vascularized human primary placental barrier model which can be constructed in 1 day. We illustrate the similarity of our model to first trimester human placenta, both in its structure and in its ability to respond to altered oxygen and to secrete factors that cause damage cells across the barrier including embryonic cortical neurons. We use this model to highlight the possibility that both the trophoblast and the endothelium within the placenta might play a role in the fetomaternal dialogue.",
keywords = "3D-tissue model, Layer-by-layer, Placenta, Signalling, Vascularization",
author = "Akihiro Nishiguchi and Catherine Gilmore and Aman Sood and Michiya Matsusaki and Gavin Collett and Dionne Tannetta and Sargent, {Ian L.} and Jennifer McGarvey and Halemani, {Nagaraj D.} and Jon Hanley and Fiona Day and Simon Grant and Catherine Murdoch-Davis and Helena Kemp and Paul Verkade and Aplin, {John D.} and Mitsuru Akashi and Case, {C. Patrick}",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.biomaterials.2018.08.025",
language = "English",
volume = "192",
pages = "140--148",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium

AU - Nishiguchi, Akihiro

AU - Gilmore, Catherine

AU - Sood, Aman

AU - Matsusaki, Michiya

AU - Collett, Gavin

AU - Tannetta, Dionne

AU - Sargent, Ian L.

AU - McGarvey, Jennifer

AU - Halemani, Nagaraj D.

AU - Hanley, Jon

AU - Day, Fiona

AU - Grant, Simon

AU - Murdoch-Davis, Catherine

AU - Kemp, Helena

AU - Verkade, Paul

AU - Aplin, John D.

AU - Akashi, Mitsuru

AU - Case, C. Patrick

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Fetal development may be compromised by adverse events at the placental interface between mother and fetus. However, it is still unclear how the communication between mother and fetus occurs through the placenta. In vitro - models of the human placental barrier, which could help our understanding and which recreate three-dimensional (3D) structures with biological functionalities and vasculatures, have not been reported yet. Here we present a 3D-vascularized human primary placental barrier model which can be constructed in 1 day. We illustrate the similarity of our model to first trimester human placenta, both in its structure and in its ability to respond to altered oxygen and to secrete factors that cause damage cells across the barrier including embryonic cortical neurons. We use this model to highlight the possibility that both the trophoblast and the endothelium within the placenta might play a role in the fetomaternal dialogue.

AB - Fetal development may be compromised by adverse events at the placental interface between mother and fetus. However, it is still unclear how the communication between mother and fetus occurs through the placenta. In vitro - models of the human placental barrier, which could help our understanding and which recreate three-dimensional (3D) structures with biological functionalities and vasculatures, have not been reported yet. Here we present a 3D-vascularized human primary placental barrier model which can be constructed in 1 day. We illustrate the similarity of our model to first trimester human placenta, both in its structure and in its ability to respond to altered oxygen and to secrete factors that cause damage cells across the barrier including embryonic cortical neurons. We use this model to highlight the possibility that both the trophoblast and the endothelium within the placenta might play a role in the fetomaternal dialogue.

KW - 3D-tissue model

KW - Layer-by-layer

KW - Placenta

KW - Signalling

KW - Vascularization

UR - http://www.scopus.com/inward/record.url?scp=85057153736&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2018.08.025

DO - 10.1016/j.biomaterials.2018.08.025

M3 - Article

C2 - 30448698

AN - SCOPUS:85057153736

VL - 192

SP - 140

EP - 148

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -