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Approach and Results: Using a membrane-tethered fluorophore reporter system, we show that EVs can be fluorescently labelled in larval and adult zebrafish and demonstrate that multiple cell types including endothelial cells and cardiomyocytes actively produce EVs in vivo. Cell-type specific EVs can be tracked by high spatiotemporal resolution light-sheet live imaging and modified flow cytometry methods allow these EVs to be further evaluated. Additionally, cryo-EM reveals the full morphological diversity of larval and adult EVs. Importantly, we demonstrate the utility of this model by showing that different cell types exchange EVs in the adult heart and that ischaemic injury models dynamically alter EV production.
Conclusions: We describe a powerful in vivo zebrafish model for the investigation of endogenous EVs in all aspects of cardiovascular biology and pathology. A cell membrane fluorophore labelling approach allows cell type-specific tracing of EV origin without bias towards the expression of individual protein markers and will allow detailed future examination of their function.
|Number of pages
|Arteriosclerosis, Thrombosis, and Vascular Biology
|Early online date
|15 Jul 2021
|Published - Sept 2021
Bibliographical notePublisher Copyright:
© 2021 Georg Thieme Verlag. All rights reserved.
- extracellular vesicle
- flow cytometry
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- 1 Finished
Scott, A., 6 Nov 2018
Student thesis: Master's Thesis › Master of Science by Research (MScR)File