In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Daisuke Sugiyama; Sung Won Hur; Anthony E. Pickering; Daisuke Kase; Sang Jeong Kim; Mikito Kawamata; Keiji Imoto; Hidemasa Furue

doi:10.1113/jphysiol.2011.226407

In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Daisuke Sugiyama, Sung Won Hur, Anthony E. Pickering, Daisuke Kase, Sang Jeong Kim, Mikito Kawamata, Keiji Imoto, Hidemasa Furue^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

33 Citations (Scopus)

Abstract

Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

Original language	English
Pages (from-to)	2225-2231
Number of pages	7
Journal	Journal of Physiology
Volume	590
Issue number	10
Early online date	13 Apr 2012
DOIs	https://doi.org/10.1113/jphysiol.2011.226407
Publication status	Published - 1 May 2012

Access to Document

10.1113/jphysiol.2011.226407

Handle.net

Persistent link

Cite this

@article{90837b4ece0b4dedb40067a6113ab0dd,

title = "In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem",

abstract = "Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.",

author = "Daisuke Sugiyama and Hur, {Sung Won} and Pickering, {Anthony E.} and Daisuke Kase and Kim, {Sang Jeong} and Mikito Kawamata and Keiji Imoto and Hidemasa Furue",

year = "2012",

month = may,

day = "1",

doi = "10.1113/jphysiol.2011.226407",

language = "English",

volume = "590",

pages = "2225--2231",

journal = "Journal of Physiology",

issn = "0022-3751",

publisher = "Wiley",

number = "10",

}

TY - JOUR

T1 - In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

AU - Sugiyama, Daisuke

AU - Hur, Sung Won

AU - Pickering, Anthony E.

AU - Kase, Daisuke

AU - Kim, Sang Jeong

AU - Kawamata, Mikito

AU - Imoto, Keiji

AU - Furue, Hidemasa

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

AB - Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

UR - http://www.scopus.com/inward/record.url?scp=84861013033&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2011.226407

DO - 10.1113/jphysiol.2011.226407

M3 - Article (Academic Journal)

C2 - 22371480

AN - SCOPUS:84861013033

SN - 0022-3751

VL - 590

SP - 2225

EP - 2231

JO - Journal of Physiology

JF - Journal of Physiology

IS - 10

ER -

In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Abstract

Access to Document

Handle.net

Other files and links

Fingerprint

Optoactivation of locus ceruleus neurons evokes bidirectional changes in thermal nociception in rats

Systemic dexmedetomidine augments inhibitory synaptic transmission in the superficial dorsal horn through activation of descending noradrenergic control: An in vivo patch-clamp analysis of analgesic mechanisms

Endogenous analgesic action of the pontospinal noradrenergic system spatially restricts and temporally delays the progression of neuropathic pain following tibial nerve injury

Cite this