In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Daisuke Sugiyama; Sung Won Hur; Anthony E. Pickering; Daisuke Kase; Sang Jeong Kim; Mikito Kawamata; Keiji Imoto; Hidemasa Furue

doi:10.1113/jphysiol.2011.226407

In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Daisuke Sugiyama
, Sung Won Hur
, Anthony E. Pickering
, Daisuke Kase
, Sang Jeong Kim
, Mikito Kawamata
, Keiji Imoto
, Hidemasa Furue^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

36 Citations (Scopus)

Abstract

Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

Original language	English
Pages (from-to)	2225-2231
Number of pages	7
Journal	Journal of Physiology
Volume	590
Issue number	10
Early online date	13 Apr 2012
DOIs	https://doi.org/10.1113/jphysiol.2011.226407
Publication status	Published - 1 May 2012

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title = "In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem",

abstract = "Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.",

author = "Daisuke Sugiyama and Hur, \{Sung Won\} and Pickering, \{Anthony E.\} and Daisuke Kase and Kim, \{Sang Jeong\} and Mikito Kawamata and Keiji Imoto and Hidemasa Furue",

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T1 - In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

AU - Sugiyama, Daisuke

AU - Hur, Sung Won

AU - Pickering, Anthony E.

AU - Kase, Daisuke

AU - Kim, Sang Jeong

AU - Kawamata, Mikito

AU - Imoto, Keiji

AU - Furue, Hidemasa

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

AB - Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

UR - https://www.scopus.com/pages/publications/84861013033

U2 - 10.1113/jphysiol.2011.226407

DO - 10.1113/jphysiol.2011.226407

M3 - Article (Academic Journal)

C2 - 22371480

AN - SCOPUS:84861013033

SN - 0022-3751

VL - 590

SP - 2225

EP - 2231

JO - Journal of Physiology

JF - Journal of Physiology

IS - 10

ER -

In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

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