In Vivo Quantification of Peroxisome Tethering to Chloroplasts in Tobacco Epidermal Cells Using Optical Tweezers

H Gao, Jeremy Metz, Nicholas A Teanby, AD Ward, SW Botchway, B Coles, MR Pollard, Imogen A Sparkes*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

32 Citations (Scopus)
315 Downloads (Pure)

Abstract

Peroxisomes are highly motile organelles that display a range of motions within a short time frame. In static snapshots, they can be juxtaposed to chloroplasts, which has led to the hypothesis that they are physically interacting. Here, using optical tweezers, we tested the dynamic physical interaction in vivo. Using near-infrared optical tweezers combined with TIRF microscopy, we were able to trap peroxisomes and approximate the forces involved in chloroplast association in vivo in tobacco (Nicotiana tabacum) and observed weaker tethering to additional unknown structures within the cell. We show that chloroplasts and peroxisomes are physically tethered through peroxules, a poorly described structure in plant cells. We suggest that peroxules have a novel role in maintaining peroxisome-organelle interactions in the dynamic environment. This could be important for fatty acid mobilization and photorespiration through the interaction with oil bodies and chloroplasts, highlighting a fundamentally important role for organelle interactions for essential biochemistry and physiological processes.
Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalPlant Physiology
Volume170
Issue number1
Early online date30 Oct 2015
DOIs
Publication statusPublished - Jan 2016

Keywords

  • Actins
  • Chloroplasts
  • Optical Tweezers
  • Peroxisomes
  • Plant Epidermis
  • Tobacco
  • Journal Article
  • Research Support, Non-U.S. Gov't

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