Induced autophagy reduces virus output in dengue infected monocytic cells

M Panyasrivanit, MP Greenwood, D Murphy, C Isidoro, P Auewarakul, DR Smith

Research output: Contribution to journalArticle (Academic Journal)

34 Citations (Scopus)

Abstract

While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susceptible to antibody enhanced infection. This study sought to determine the involvement of autophagy in the DENV infection of monocytic cells, using U937 cells as a model system. While the induction of autophagy was seen in response to DENV-2 infection, biochemical induction of autophagy resulted in a significant decrease in virus output. Down regulation of autophagy resulted in only a very slight increase in intracellular virus levels. In monocytic cells autophagy is not a significant part of the DENV replication mechanism, and there are distinct cell type specific differences in the DENV-autophagy interaction.
Translated title of the contributionInduced autophagy reduces virus output in dengue infected monocytic cells
Original languageEnglish
Pages (from-to)74 - 84
Number of pages11
JournalVirology
Volume15;418(1)
DOIs
Publication statusPublished - Sep 2011

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