Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus

M Todoroki, Y Ueta, H Fujihara, H Otsubo, M Shibata, H Hashimoto, M Kobayashi, H Sakamoto, M Kawata, G Dayanithi, D Murphy, H Hiro, K Takahashi, S Nagata

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Abstract

We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 12–48 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress. Read More: http://informahealthcare.com/doi/abs/10.3109/10253890903383406
Translated title of the contributionInduction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus
Original languageEnglish
Pages (from-to)281 - 291
Number of pages11
JournalStress
Volume13(4)
DOIs
Publication statusPublished - Jul 2010

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    Todoroki, M., Ueta, Y., Fujihara, H., Otsubo, H., Shibata, M., Hashimoto, H., Kobayashi, M., Sakamoto, H., Kawata, M., Dayanithi, G., Murphy, D., Hiro, H., Takahashi, K., & Nagata, S. (2010). Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus. Stress, 13(4), 281 - 291. https://doi.org/10.3109/10253890903383406