Inexpensive and fast pathogenic bacteria screening using field-effect transistors

Nello Formisano, Nikhil Bhalla, Mel Heeran, Juana Reyes Martinez, Amrita Sarkar, Maisem Laabei, Pawan Jolly, Chris R. Bowen, John T Taylor, Sabine Flitsch, Pedro Estrela*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

30 Citations (Scopus)

Abstract

While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that often involve culturing which can be time-consuming. Therefore, innovative, simple, rapid and low-cost solutions to effectively reduce the burden of bacterial infections are urgently needed. Here we demonstrate a label-free sensor for fast bacterial detection based on metal-oxide-semiconductor field-effect transistors (MOSFETs). The electric charge of bacteria binding to the glycosylated gates of a MOSFET enables quantification in a straightforward manner. We show that the limit of quantitation is 1.9×10(5) CFU/mL with this simple device, which is more than 10,000-times lower than is achieved with electrochemical impedance spectroscopy (EIS) and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF) on the same modified surfaces. Moreover, the measurements are extremely fast and the sensor can be mass produced at trivial cost as a tool for initial screening of pathogens.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalBiosensors and Bioelectronics
Volume85
Early online date21 Apr 2016
DOIs
Publication statusPublished - 15 Nov 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.

Keywords

  • Bacteria/isolation & purification
  • Bacterial Infections/diagnosis
  • Bacteriological Techniques/economics
  • Biosensing Techniques/economics
  • Dielectric Spectroscopy
  • Equipment Design
  • Escherichia coli/isolation & purification
  • Escherichia coli Infections/diagnosis
  • Humans
  • Semiconductors/economics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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