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Injury activates a dynamic cytoprotective network to confer stress resilience and drive repair

Research output: Contribution to journalArticle

Original languageEnglish
Number of pages17
JournalCurrent Biology
Volume29
DOIs
DateAccepted/In press - 13 Sep 2019
DatePublished (current) - 24 Oct 2019

Abstract

In healthy individuals, injured tissues rapidly repair themselves following damage. Within a healing skin wound, recruited inflammatory cells release a multitude of bacteriocidal factors, including reactive oxygen species (ROS), to eliminate invading pathogens. Paradoxically, whilst these highly reactive ROS confer resistance to infection, they are also toxic to host tissues and may ultimately delay repair. Repairing tissues have therefore evolved powerful cytoprotective ‘resilience’ machinery to protect against and tolerate this collateral damage. Here, we use in vivo time-lapse imaging and genetic manipulation in Drosophila to dissect the molecular and cellular mechanisms that drive tissue resilience to wound-induced stress. We identify a dynamic, cross-regulatory network of stress-activated cytoprotective pathways, linking calcium, JNK, Nrf2 and Gadd45, that act to both ‘shield’ tissues from oxidative damage and promote efficient damage repair. Ectopic activation of these pathways confers stress protection to naïve tissue, whilst their inhibition leads to marked delays in wound closure. Strikingly, the induction of cytoprotection is tightly linked to the pathways that initiate the inflammatory response, suggesting evolution of a fail-safe mechanism for tissue protection each time inflammation is triggered. A better understanding of these resilience mechanisms - their identities and precise spatio-temporal regulation - is of major clinical importance for development of therapeutic interventions for all pathologies linked to oxidative stress, including debilitating chronic non-healing wounds.

    Research areas

  • inflammation, tissue repair, tissue resilience, cytoprotection, oxidative stress, ROS, DNA damage, hormesis, wound, preconditioning

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Elsevier at https://www.sciencedirect.com/science/article/pii/S0960982219311972 . Please refer to any applicable terms of use of the publisher.

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