Insulin-dependent GLUT4 trafcking is not regulated by protein SUMOylation in L6 myocytes

Ruth Ellen Carmichael, Kevin Wilkinson, Tim Craig*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

1 Citation (Scopus)
132 Downloads (Pure)

Abstract

Type-II Diabetes Mellitus (T2DM) is one of the fastest growing public health issues today, consuming 12% of worldwide health budgets and affecting an estimated 400 million people. One of the key pathological traits of this disease is insulin resistance at ‘glucose sink’ tissues (mostly skeletal muscle), and this remains one of the features of this disease most intractable to therapeutic intervention. Several lines of evidence have implicated the post-translational modification, SUMOylation, in insulin signalling and insulin resistance in skeletal muscle. In this study, we examined this possibility by manipulation of cellular SUMOylation levels using multiple different tools, and assaying the effect on insulin-stimulated GLUT4 surface expression in differentiated L6 rat myocytes. Although insulin stimulation of L6 myocytes produced a robust decrease in total cellular SUMO1-ylation levels, manipulating cellular SUMOylation had no effect on insulin-responsive GLUT4 surface trafficking using any of the tools we employed. Whilst we cannot totally exclude the possibility that SUMOylation plays a role in the insulin signalling pathway in human health and disease, our data strongly argue that GLUT4 trafficking in response to insulin is not regulated by protein SUMOylation, and that SUMOylation does not therefore represent a viable therapeutic target for the treatment of insulin resistance.
Original languageEnglish
Article number6477
Number of pages9
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 24 Apr 2019

Fingerprint Dive into the research topics of 'Insulin-dependent GLUT4 trafcking is not regulated by protein SUMOylation in L6 myocytes'. Together they form a unique fingerprint.

Cite this