Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease

Oona Y-C. Lee; Ian D Bull; E Molnar; A  Marcsik; G  Palfi; Helen D. Donoghue; Gurdyal S. Besra; David E. Minnikin

Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease

Oona Y-C. Lee, Ian D Bull, E Molnar, A Marcsik, G Palfi, Helen D. Donoghue, Gurdyal S. Besra, David E. Minnikin

Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

Abstract

The use of ancient DNA and lipid biomarkers has become established for the detection of tuberculosis and leprosy in archaeological material. Long-chain compounds are released by an efficient non-aqueous alkaline extraction and acidic components are converted to stable pentafluorobenzyl (PFB) esters, which can be preserved for immediate or future analysis. These long-chain components are fractionated into nonhydroxylated fatty acid esters and mycolic acid esters. Characteristic profiles of mycolates are recorded by fluorescence high-performance liquid chromatography (HPLC) of pyrenebutyric acid derivatives of PFB esters. Mycocerosate PFB esters are analysed by negative-ion chemical-ionisation gas chromatography-mass spectrometry (NICI-GCMS). In this study, ancient DNA analyses, along with mycolate and mycocerosate profiles, for a representative late 7th century AD skeleton from the Avar period in Hungary, are presented. It will be shown how mycolate and mycocerosate analyses of residual material from an ancient DNA analysis can be used to confirm a mixed leprosy-tuberculosis infection and provide an estimate of the relative amounts of the M. tuberculosis and M. leprae bacterial load obtained. In the present case, the bone pathology indicated only leprosy, but the biomarker analysis suggested predominance of tuberculosis over leprosy.

Original language	English
Title of host publication	Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology
Subtitle of host publication	Department of Archaeology and Anthropology University of Cambridge 2010
Editors	Piers D. Mitchell, Jo Buckberry
Pages	63-69
Number of pages	7
Publication status	Published - 2012

Publication series

Name	British Archaeological Reports International Series
Publisher	BAR Publishing
Volume	2380
ISSN (Print)	0143-3067

Keywords

Tuberculosis
Paleopathology
Skeletons
Lipids
Biomarkers
Leprosy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Lee, O. Y.-C., Bull, I. D., Molnar, E., Marcsik, A., Palfi, G., Donoghue, H. D., Besra, G. S., & Minnikin, D. E. (2012). Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease. In P. D. Mitchell, & J. Buckberry (Eds.), Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology: Department of Archaeology and Anthropology University of Cambridge 2010 (pp. 63-69). (British Archaeological Reports International Series; Vol. 2380). http://www.barpublishing.com/proceedings-of-the-twelfth-annual-conference-of-the-british-association-for-biological-anthropology-and-osteoarchaeology.html

Lee, Oona Y-C. ; Bull, Ian D ; Molnar, E et al. / Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease. Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology: Department of Archaeology and Anthropology University of Cambridge 2010. editor / Piers D. Mitchell ; Jo Buckberry. 2012. pp. 63-69 (British Archaeological Reports International Series).

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title = "Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease",

abstract = "The use of ancient DNA and lipid biomarkers has become established for the detection of tuberculosis and leprosy in archaeological material. Long-chain compounds are released by an efficient non-aqueous alkaline extraction and acidic components are converted to stable pentafluorobenzyl (PFB) esters, which can be preserved for immediate or future analysis. These long-chain components are fractionated into nonhydroxylated fatty acid esters and mycolic acid esters. Characteristic profiles of mycolates are recorded by fluorescence high-performance liquid chromatography (HPLC) of pyrenebutyric acid derivatives of PFB esters. Mycocerosate PFB esters are analysed by negative-ion chemical-ionisation gas chromatography-mass spectrometry (NICI-GCMS). In this study, ancient DNA analyses, along with mycolate and mycocerosate profiles, for a representative late 7th century AD skeleton from the Avar period in Hungary, are presented. It will be shown how mycolate and mycocerosate analyses of residual material from an ancient DNA analysis can be used to confirm a mixed leprosy-tuberculosis infection and provide an estimate of the relative amounts of the M. tuberculosis and M. leprae bacterial load obtained. In the present case, the bone pathology indicated only leprosy, but the biomarker analysis suggested predominance of tuberculosis over leprosy. ",

keywords = "Tuberculosis, Paleopathology , Skeletons, Lipids, Biomarkers, Leprosy",

author = "Lee, {Oona Y-C.} and Bull, {Ian D} and E Molnar and A Marcsik and G Palfi and Donoghue, {Helen D.} and Besra, {Gurdyal S.} and Minnikin, {David E.}",

year = "2012",

language = "English",

isbn = "9781407309705",

series = "British Archaeological Reports International Series",

publisher = "BAR Publishing",

pages = "63--69",

editor = "Mitchell, {Piers D.} and Jo Buckberry",

booktitle = "Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology",

}

Lee, OY-C, Bull, ID, Molnar, E, Marcsik, A, Palfi, G, Donoghue, HD, Besra, GS & Minnikin, DE 2012, Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease. in PD Mitchell & J Buckberry (eds), Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology: Department of Archaeology and Anthropology University of Cambridge 2010. British Archaeological Reports International Series, vol. 2380, pp. 63-69. <http://www.barpublishing.com/proceedings-of-the-twelfth-annual-conference-of-the-british-association-for-biological-anthropology-and-osteoarchaeology.html>

Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease. / Lee, Oona Y-C.; Bull, Ian D; Molnar, E et al.
Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology: Department of Archaeology and Anthropology University of Cambridge 2010. ed. / Piers D. Mitchell; Jo Buckberry. 2012. p. 63-69 (British Archaeological Reports International Series; Vol. 2380).

Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

TY - GEN

T1 - Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease

AU - Lee, Oona Y-C.

AU - Bull, Ian D

AU - Molnar, E

AU - Marcsik, A

AU - Palfi, G

AU - Donoghue, Helen D.

AU - Besra, Gurdyal S.

AU - Minnikin, David E.

PY - 2012

Y1 - 2012

N2 - The use of ancient DNA and lipid biomarkers has become established for the detection of tuberculosis and leprosy in archaeological material. Long-chain compounds are released by an efficient non-aqueous alkaline extraction and acidic components are converted to stable pentafluorobenzyl (PFB) esters, which can be preserved for immediate or future analysis. These long-chain components are fractionated into nonhydroxylated fatty acid esters and mycolic acid esters. Characteristic profiles of mycolates are recorded by fluorescence high-performance liquid chromatography (HPLC) of pyrenebutyric acid derivatives of PFB esters. Mycocerosate PFB esters are analysed by negative-ion chemical-ionisation gas chromatography-mass spectrometry (NICI-GCMS). In this study, ancient DNA analyses, along with mycolate and mycocerosate profiles, for a representative late 7th century AD skeleton from the Avar period in Hungary, are presented. It will be shown how mycolate and mycocerosate analyses of residual material from an ancient DNA analysis can be used to confirm a mixed leprosy-tuberculosis infection and provide an estimate of the relative amounts of the M. tuberculosis and M. leprae bacterial load obtained. In the present case, the bone pathology indicated only leprosy, but the biomarker analysis suggested predominance of tuberculosis over leprosy.

AB - The use of ancient DNA and lipid biomarkers has become established for the detection of tuberculosis and leprosy in archaeological material. Long-chain compounds are released by an efficient non-aqueous alkaline extraction and acidic components are converted to stable pentafluorobenzyl (PFB) esters, which can be preserved for immediate or future analysis. These long-chain components are fractionated into nonhydroxylated fatty acid esters and mycolic acid esters. Characteristic profiles of mycolates are recorded by fluorescence high-performance liquid chromatography (HPLC) of pyrenebutyric acid derivatives of PFB esters. Mycocerosate PFB esters are analysed by negative-ion chemical-ionisation gas chromatography-mass spectrometry (NICI-GCMS). In this study, ancient DNA analyses, along with mycolate and mycocerosate profiles, for a representative late 7th century AD skeleton from the Avar period in Hungary, are presented. It will be shown how mycolate and mycocerosate analyses of residual material from an ancient DNA analysis can be used to confirm a mixed leprosy-tuberculosis infection and provide an estimate of the relative amounts of the M. tuberculosis and M. leprae bacterial load obtained. In the present case, the bone pathology indicated only leprosy, but the biomarker analysis suggested predominance of tuberculosis over leprosy.

KW - Tuberculosis

KW - Paleopathology

KW - Skeletons

KW - Lipids

KW - Biomarkers

KW - Leprosy

M3 - Conference Contribution (Conference Proceeding)

SN - 9781407309705

T3 - British Archaeological Reports International Series

SP - 63

EP - 69

BT - Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology

A2 - Mitchell, Piers D.

A2 - Buckberry, Jo

ER -

Lee OYC, Bull ID, Molnar E, Marcsik A, Palfi G, Donoghue HD et al. Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease. In Mitchell PD, Buckberry J, editors, Proceedings of the Twelfth Annual Conference of the British Association for Biological Anthropology and Osteoarchaeology: Department of Archaeology and Anthropology University of Cambridge 2010. 2012. p. 63-69. (British Archaeological Reports International Series).

Integrated Strategies for the use of Lipid Biomarkers in the Diagnosis of Ancient Mycobacterial Disease

Abstract

Publication series

Keywords

UN SDGs

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