Interaction between prion protein and toxic amyloid β assemblies can be therapeutically targeted at multiple sites

D.B Freir, A.J Nicoll, I Klyubin, S Panico, J.M McDonald, E Risse, E.A Asante, M.A Farrow, R.B Sessions, H.R Saibil, A.R Clarke, M.J Rowan, D.M Walsh, J Collinge

Research output: Contribution to journalArticle (Academic Journal)peer-review

209 Citations (Scopus)

Abstract

A role for PrP in the toxic effect of oligomeric forms of Aβ, implicated in Alzheimer’s disease (AD), has been suggested but remains controversial. Here we show that PrP is required for the plasticity-impairing effects of ex vivo material from human AD brain and that standardized Aβ-derived diffusible ligand (ADDL) preparations disrupt hippocampal synaptic plasticity in a PrP-dependent manner. We screened a panel of anti-PrP antibodies for their ability to disrupt the ADDL–PrP interaction. Antibodies directed to the principal PrP/Aβ-binding site and to PrP helix-1, were able to block Aβ binding to PrP suggesting that the toxic Aβ species are of relatively high molecular mass and/or may bind multiple PrP molecules. Two representative and extensively characterized monoclonal antibodies directed to these regions, ICSM-35 and ICSM-18, were shown to block the Aβ-mediated disruption of synaptic plasticity validating these antibodies as candidate therapeutics for AD either individually or in combination.
Translated title of the contributionInteraction between prion protein and toxic amyloid β assemblies can be therapeutically targeted at multiple sites
Original languageEnglish
Pages (from-to)336-1 - 336-9
Number of pages9
JournalNature Communications
Volume2
DOIs
Publication statusPublished - Jun 2011

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