Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos

Iwan R Evans, Nan Hu, Helen Skaer, Will Wood

Research output: Contribution to journalArticle (Academic Journal)

29 Citations (Scopus)

Abstract

During embryonic development, Drosophila macrophages (haemocytes) undergo a series of stereotypical migrations to disperse throughout the embryo. One major migratory route is along the ventral nerve cord (VNC), where haemocytes are required for the correct development of this tissue. We show, for the first time, that a reciprocal relationship exists between haemocytes and the VNC and that defects in nerve cord development prevent haemocyte migration along this structure. Using live imaging, we demonstrate that the axonal guidance cue Slit and its receptor Robo are both required for haemocyte migration, but signalling is not autonomously required in haemocytes. We show that the failure of haemocyte migration along the VNC in slit mutants is not due to a lack of chemotactic signals within this structure, but rather to a failure in its detachment from the overlying epithelium, creating a physical barrier to haemocyte migration. This block of haemocyte migration in turn disrupts the formation of the dorsoventral channels within the VNC, further highlighting the importance of haemocyte migration for correct neural development. This study illustrates the important role played by the three-dimensional environment in directing cell migration in vivo and reveals an intriguing interplay between the developing nervous system and the blood cells within the fly, demonstrating that their development is both closely coupled and interdependent.

Original languageEnglish
Pages (from-to)1625-33
Number of pages9
JournalDevelopment (Cambridge)
Volume137
Issue number10
DOIs
Publication statusPublished - May 2010

Keywords

  • Animals
  • Animals, Genetically Modified
  • Body Patterning
  • Cell Movement
  • Drosophila
  • Drosophila Proteins
  • Embryo, Nonmammalian
  • Embryonic Development
  • Hemocytes
  • Macrophages
  • Models, Biological
  • Nerve Tissue Proteins
  • Nervous System
  • Receptors, Immunologic
  • Signal Transduction

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