Interfacial Assembly of Protein-polymer Nano-conjugates into Stimulus-Responsive Biomimetic Protocells

Xin Huang, Mei Li, David C Green, David S Williams, Avinash J Patil, Stephen Mann

Research output: Contribution to journalArticle (Academic Journal)

217 Citations (Scopus)
480 Downloads (Pure)

Abstract

The mechanism of spontaneous assembly of microscale compartments is a central question for the origin of life, and has technological repercussions in diverse areas such as materials science, catalysis, biotechnology and biomedicine. Such compartments need to be semi-permeable, structurally robust and capable of housing assemblages of functional components for internalized chemical transformations. In principle, proteins should be ideal building blocks for the construction of membrane-bound compartments but protein vesicles with cell-like properties are extremely rare. Here we present an approach to the interfacial assembly of protein-based micro-compartments (proteinosomes) that are delineated by a semi-permeable, stimulus-responsive, enzymatically active, elastic membrane consisting of a closely packed monolayer of conjugated protein–polymer building blocks. The proteinosomes can be dispersed in oil or water, thermally cycled to temperatures of 70 °C, and partially dried and re-inflated without loss of structural integrity. As a consequence, they exhibit protocellular properties such as guest molecule encapsulation, selective permeability, gene-directed protein synthesis and membrane-gated internalized enzyme catalysis.
Original languageEnglish
Article number2239
Number of pages9
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 30 Jul 2013

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