Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy

Aarti Kathrani*, Victor Lezcano, Edward J Hall, Albert E Jergens, Yeon-Jung Seo, Jonathan P Mochel, Todd Atherly, Karin Allenspach

*Corresponding author for this work

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Abstract

Background: A recent genome-wide association study in German Shepherd dogs (GSDs) with chronic enteropathy (CE) has identified polymorphisms in the Th2 cytokine genes. Hypothesis/objective: To determine if the expression of the Th2 cytokines, interleukin-13 (IL-13) and interleukin-33 (IL-33), is altered in the duodenal mucosa of GSDs with CE compared to non-GSDs with CE and healthy dogs. Animals: Twenty client-owned dogs diagnosed with CE (10 GSDs and 10 non-GSDs) at the Bristol Veterinary School and 8 healthy Beagle dogs from the Iowa State University Service Colony. Methods: Retrospective study using archived paraffin-embedded duodenal biopsy samples. A novel RNA in situ hybridization technology (RNAscope) was used to hybridize IL-13 and IL-33 mRNA probes onto at least 10 sections from duodenal biopsy samples for each dog. RNAscope signals were visualized using a microscope and semi-quantitative assessment was performed by a single operator. Results: Based on duodenal villus, subvillus, epithelial, and lamina propria average expression scores, GSDs with CE had significantly lower IL-13 and IL-33 mRNA expression compared to non-GSDs with CE (IL-13, P <.04; IL-33, P <.02) and healthy Beagle dogs (IL-13, P <.02; IL-33, P <.004). Conclusions and Clinical Importance: Similar to human patients with ulcerative colitis, a subtype of human inflammatory bowel disease, these data indicate that Th2 cytokines may be involved in the pathogenesis of CE in GSDs.

Original languageEnglish
Pages (from-to)1660-1668
Number of pages9
JournalJournal of Veterinary Internal Medicine
Volume33
Issue number4
Early online date6 Jun 2019
DOIs
Publication statusPublished - 1 Jul 2019

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Keywords

  • bowel
  • canine
  • cytokine
  • duodenum
  • inflammatory
  • Th2

Cite this

Kathrani, A., Lezcano, V., Hall, E. J., Jergens, A. E., Seo, Y-J., Mochel, J. P., Atherly, T., & Allenspach, K. (2019). Interleukin-13 and interleukin-33 mRNA are underexpressed in the duodenal mucosa of German Shepherd dogs with chronic enteropathy. Journal of Veterinary Internal Medicine, 33(4), 1660-1668. https://doi.org/10.1111/jvim.15544