Investigation of Atherosclerotic Plaque Vulnerability

Sarah J George; Jason L Johnson

doi:10.1007/978-1-0716-1924-7_32

Investigation of Atherosclerotic Plaque Vulnerability

Sarah J George
, Jason L Johnson

Bristol Medical School (THS)

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

3 Citations (Scopus)

Abstract

Histochemical and immunohistochemical approaches permit the detection and evaluation of proteins and cell types within murine brachiocephalic artery atherosclerotic plaques, that can be subsequently analyzed to provide inferences on atherosclerotic plaque vulnerability. Here we describe the specific histochemical techniques deployed to examine the expression of elastin, fibrillar collagens, and neutral lipids, alongside immunohistochemistry protocols for the identification of macrophages (CD68) and vascular smooth muscle cells (α-smooth muscle actin). We will also describe how analyses derived from these methods can be combined to determine evidence of previous plaque rupture and susceptibility to rupture.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	521-535
Number of pages	15
Volume	2419
DOIs	https://doi.org/10.1007/978-1-0716-1924-7_32
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2419
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
Jason Johnson is funded by British Heart Foundation Senior Research Fellowship, FS/18/1/33234.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Research Groups and Themes

Bristol Heart Institute

Keywords

Animals
Immunohistochemistry
Macrophages/metabolism
Mice
Myocytes, Smooth Muscle/metabolism
Plaque, Atherosclerotic/metabolism

Access to Document

10.1007/978-1-0716-1924-7_32

Handle.net

Persistent link

Pre-clinical evaluation of MMP-12 inhibition for the prevention and treatment of abdominal aortic aneurysm formation and progression
Johnson, J. L. (Principal Investigator)
21/08/15 → 20/08/17
Project: Research

Cite this

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title = "Investigation of Atherosclerotic Plaque Vulnerability",

abstract = "Histochemical and immunohistochemical approaches permit the detection and evaluation of proteins and cell types within murine brachiocephalic artery atherosclerotic plaques, that can be subsequently analyzed to provide inferences on atherosclerotic plaque vulnerability. Here we describe the specific histochemical techniques deployed to examine the expression of elastin, fibrillar collagens, and neutral lipids, alongside immunohistochemistry protocols for the identification of macrophages (CD68) and vascular smooth muscle cells (α-smooth muscle actin). We will also describe how analyses derived from these methods can be combined to determine evidence of previous plaque rupture and susceptibility to rupture.",

keywords = "Animals, Immunohistochemistry, Macrophages/metabolism, Mice, Myocytes, Smooth Muscle/metabolism, Plaque, Atherosclerotic/metabolism",

author = "George, \{Sarah J\} and Johnson, \{Jason L\}",

note = "Funding Information: Jason Johnson is funded by British Heart Foundation Senior Research Fellowship, FS/18/1/33234. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-1924-7\_32",

language = "English",

volume = "2419",

series = "Methods in Molecular Biology",

pages = "521--535",

booktitle = "Methods in Molecular Biology",

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T1 - Investigation of Atherosclerotic Plaque Vulnerability

AU - George, Sarah J

AU - Johnson, Jason L

N1 - Funding Information: Jason Johnson is funded by British Heart Foundation Senior Research Fellowship, FS/18/1/33234. Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Histochemical and immunohistochemical approaches permit the detection and evaluation of proteins and cell types within murine brachiocephalic artery atherosclerotic plaques, that can be subsequently analyzed to provide inferences on atherosclerotic plaque vulnerability. Here we describe the specific histochemical techniques deployed to examine the expression of elastin, fibrillar collagens, and neutral lipids, alongside immunohistochemistry protocols for the identification of macrophages (CD68) and vascular smooth muscle cells (α-smooth muscle actin). We will also describe how analyses derived from these methods can be combined to determine evidence of previous plaque rupture and susceptibility to rupture.

AB - Histochemical and immunohistochemical approaches permit the detection and evaluation of proteins and cell types within murine brachiocephalic artery atherosclerotic plaques, that can be subsequently analyzed to provide inferences on atherosclerotic plaque vulnerability. Here we describe the specific histochemical techniques deployed to examine the expression of elastin, fibrillar collagens, and neutral lipids, alongside immunohistochemistry protocols for the identification of macrophages (CD68) and vascular smooth muscle cells (α-smooth muscle actin). We will also describe how analyses derived from these methods can be combined to determine evidence of previous plaque rupture and susceptibility to rupture.

KW - Animals

KW - Immunohistochemistry

KW - Macrophages/metabolism

KW - Mice

KW - Myocytes, Smooth Muscle/metabolism

KW - Plaque, Atherosclerotic/metabolism

U2 - 10.1007/978-1-0716-1924-7_32

DO - 10.1007/978-1-0716-1924-7_32

M3 - Chapter in a book

C2 - 35237986

VL - 2419

T3 - Methods in Molecular Biology

SP - 521

EP - 535

BT - Methods in Molecular Biology

ER -

Investigation of Atherosclerotic Plaque Vulnerability

Abstract

Publication series

Bibliographical note

Research Groups and Themes

Keywords

Access to Document

Handle.net

Fingerprint

Projects

Pre-clinical evaluation of MMP-12 inhibition for the prevention and treatment of abdominal aortic aneurysm formation and progression

Cite this