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Abstract
A new N-benzenesulfonyl-based ionic-liquid mass spectroscopy label (I-Tag2) for covalent attachment to substrates has been prepared. I-Tag2 was used to monitor oligosaccharide elongation and serve as a purification handle. Starting from chemically synthesized I-Tag2-labelled N-acetyl glucosamine (GlcNAc) 1, I-Tag2-LacNAc (Gal beta(1-4)GlcNAc) 2 and I-Tag2-Lewis(X) (Gal beta(1-4)[Fuc alpha(1-3)]GlcNAc) 3, which are oligosaccharides of biological relevance, were enzymatically prepared. The apparent kinetic parameters for the enzyme catalysed transformations with beta-1,4-galactosyltransferase (beta-1,4-GalT) and fucosyltransferase VI (FucT VI) were measured by LC-MS demonstrating the applicability and versatility of the new I-Tags in enzymatic transformations with glycosyltransferases.
Original language | English |
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Pages (from-to) | 7091-7097 |
Number of pages | 7 |
Journal | Organic and Biomolecular Chemistry |
Volume | 10 |
Issue number | 35 |
DOIs | |
Publication status | Published - 21 Sept 2012 |
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Dive into the research topics of 'Ionic-liquid-based MS probes for the chemo-enzymatic synthesis of oligosaccharides'. Together they form a unique fingerprint.Projects
- 1 Finished
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Reworking of Novel tools for Glycoscience
Galan, M. C. (Principal Investigator)
31/03/12 → 30/08/17
Project: Research