Isolation and characterisation of Mauritius lowland day gecko Phelsuma guimbeaui microsatellite loci

Steeves Buckland, Gavin Horsburgh, Deborah Dawson, Nik Cole, Andrew Krupa, Laura Gallagher, Sion Henshaw, Jim Groombridge, Stephen Harris

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)

Abstract

We isolated 315 sequences from a Phelsuma
guimbeaui microsatellite-enriched genomic library. Primer
sets were designed for 44 loci and used to genotype 29
unrelated individuals belonging to a population in the west
of Mauritius. All the loci were polymorphic and the
number of alleles ranged from 6 to 34. Mean observed and
expected heterozygosity varied from 0.29 to 1.00 and 0.48
to 0.97, respectively. Thirteen loci displayed evidence of
deviation from Hardy–Weinberg equilibrium, of which 11
also had an estimated null allele frequency C10 %. The
microsatellite loci will be used to evaluate the population
structure and genetic diversity of P. guimbeaui in
Mauritius.

Original languageEnglish
Number of pages6
JournalConservation Genet Resour
Early online date22 May 2013
DOIs
Publication statusPublished - 2013

Keywords

  • Conservation
  • Gekkonidae
  • Microsatelite marker
  • Phelsuma
  • Reptile
  • Simple sequence repeates (SSRs)

Fingerprint Dive into the research topics of 'Isolation and characterisation of Mauritius lowland day gecko <em>Phelsuma guimbeaui</em> microsatellite loci'. Together they form a unique fingerprint.

Cite this