Isolation and characterisation of Mauritius lowland day gecko Phelsuma guimbeaui microsatellite loci

We isolated 315 sequences from a Phelsuma
guimbeaui microsatellite-enriched genomic library. Primer
sets were designed for 44 loci and used to genotype 29
unrelated individuals belonging to a population in the west
of Mauritius. All the loci were polymorphic and the
number of alleles ranged from 6 to 34. Mean observed and
expected heterozygosity varied from 0.29 to 1.00 and 0.48
to 0.97, respectively. Thirteen loci displayed evidence of
deviation from Hardy–Weinberg equilibrium, of which 11
also had an estimated null allele frequency C10 %. The
microsatellite loci will be used to evaluate the population
structure and genetic diversity of P. guimbeaui in
Mauritius.