TY - JOUR
T1 - Isolation and characterization of human interleukin-10-secreting T cells from peripheral blood
AU - Mazza, Graziella
AU - Sabatos-Peyton, Catherine A.
AU - Protheroe, Rachel E.
AU - Herman, Andrew
AU - Campbell, John D.
AU - Wraith, David C.
PY - 2010/3/1
Y1 - 2010/3/1
N2 - Recent studies have expanded our understanding of the role of the anti-inflammatory cytokine interleukin (IL)-10, produced by multiple lineages of both human and murine T cells, in regulating the immune response. Here, we demonstrate that the small percentage of circulating CD4+ T cells that secrete IL-10 can be isolated from human peripheral blood and, importantly, we have optimized a protocol to expand these cells in both antigen-specific and polyclonal manners. Expanded CD4+IL-10+ T cells abrogate proliferation and T helper (Th) 1-like cytokine production in an antigen-specific manner, and to a lesser extent exhibit bystander suppressive capacity. CD4+IL-10+ T cells are suppressive in a cell contact-dependent way, though they do not require secretion of IL-10 for their suppressive role in vitro. CD4+IL-10+ T cells have an activated phenotype, with high expression of CD25, CD69, and cytotoxic T-lymphocyte antigen-4, and are largely FoxP3 negative. This novel method for the isolation and expansion of suppressive IL-10-secreting T cells has important implications both for further research and clinical therapeutic development.
AB - Recent studies have expanded our understanding of the role of the anti-inflammatory cytokine interleukin (IL)-10, produced by multiple lineages of both human and murine T cells, in regulating the immune response. Here, we demonstrate that the small percentage of circulating CD4+ T cells that secrete IL-10 can be isolated from human peripheral blood and, importantly, we have optimized a protocol to expand these cells in both antigen-specific and polyclonal manners. Expanded CD4+IL-10+ T cells abrogate proliferation and T helper (Th) 1-like cytokine production in an antigen-specific manner, and to a lesser extent exhibit bystander suppressive capacity. CD4+IL-10+ T cells are suppressive in a cell contact-dependent way, though they do not require secretion of IL-10 for their suppressive role in vitro. CD4+IL-10+ T cells have an activated phenotype, with high expression of CD25, CD69, and cytotoxic T-lymphocyte antigen-4, and are largely FoxP3 negative. This novel method for the isolation and expansion of suppressive IL-10-secreting T cells has important implications both for further research and clinical therapeutic development.
KW - Induced regulatory T cells
KW - Interleukin-10
UR - http://www.scopus.com/inward/record.url?scp=76949106053&partnerID=8YFLogxK
U2 - 10.1016/j.humimm.2009.12.003
DO - 10.1016/j.humimm.2009.12.003
M3 - Article (Academic Journal)
C2 - 20034527
AN - SCOPUS:76949106053
VL - 71
SP - 225
EP - 234
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 3
ER -