Isolation and characterization of microsatellite loci in Senecio

G Liu; MJ Hegarty; KJ Edwards; SJ Hiscock; RJ Abbott

doi:10.1111/j.1477-8286.2004.00753.x

Isolation and characterization of microsatellite loci in Senecio

G Liu, MJ Hegarty, KJ Edwards, SJ Hiscock, RJ Abbott

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

Translated title of the contribution	Isolation and characterization of microsatellite loci in Senecio
Original language	English
Pages (from-to)	611-614
Number of pages	4
Journal	Molecular Ecology Notes
Volume	4
Issue number	4
DOIs	https://doi.org/10.1111/j.1477-8286.2004.00753.x
Publication status	Published - Dec 2004

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@article{b35c1993a8d648c4b8f6eecf6ccf5ba0,

title = "Isolation and characterization of microsatellite loci in Senecio",

abstract = "Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.",

author = "G Liu and MJ Hegarty and KJ Edwards and SJ Hiscock and RJ Abbott",

year = "2004",

month = dec,

doi = "10.1111/j.1477-8286.2004.00753.x",

language = "English",

volume = "4",

pages = "611--614",

journal = "Molecular Ecology Notes",

issn = "1471-8286",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Isolation and characterization of microsatellite loci in Senecio

AU - Liu, G

AU - Hegarty, MJ

AU - Edwards, KJ

AU - Hiscock, SJ

AU - Abbott, RJ

PY - 2004/12

Y1 - 2004/12

N2 - Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

AB - Interspecific hybridization has resulted in the recent origin of several hybrid Senecio taxa at diploid, tetraploid and hexaploid levels. As part of research aimed at constructing and comparing genomic maps of each of these taxa and their parents, we have isolated micro-satellite loci from genomic DNA libraries of S. vulgaris and S. squalidus. Primers of 35 loci amplified microsatellites resolved in agarose gels from one or more of S. vulgaris, S. squalidus, S. aethnensis and S. chrysanthemifolius. Approximately 71% of primers amplified a product in all four species. A survey of microsatellite variation in S. chrysanthemifolius over a subset of 14 loci resolved 2-11 alleles per locus in polyacrylamide gels with expected heterozygosity (HE) ranging from 0.26 to 0.87.

U2 - 10.1111/j.1477-8286.2004.00753.x

DO - 10.1111/j.1477-8286.2004.00753.x

M3 - Article (Academic Journal)

SN - 1471-8286

VL - 4

SP - 611

EP - 614

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 4

ER -

Isolation and characterization of microsatellite loci in Senecio

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