Abstract
Imaging is one of the key technologies underpinning discoveries in biomedical research. Each imaging technique however usually only provides a specific type of information. For instance, live-cell imaging using fluorescent tags can show us the dynamics of a system. On the other hand, Electron Microscopy (EM) gives us better resolution combined with the structural reference space. By applying a combination of light and electron microscopy modalities to a single sample one can exploit the advantages of both techniques in Correlative Light Electron Microscopy (CLEM).
Although CLEM approaches can generate additional insights into the sample that can’t be gained by either technique in isolation, the visualisation of the object of interest via markers or probes is still one of the bottlenecks in a Correlative Microscopy workflow. Whereas fluorescence is not directly visible in a standard electron microscope, gold particles, as the most common choice of probe for EM can also only be visualised using specialised light microscopes.
In this review we will discuss some of the latest developments of probes for Correlative Light Electron Microscopy and some strategies how to choose a probe, discussing pros and cons of specific probes, and ensuring that they function as a dual modality marker.
Although CLEM approaches can generate additional insights into the sample that can’t be gained by either technique in isolation, the visualisation of the object of interest via markers or probes is still one of the bottlenecks in a Correlative Microscopy workflow. Whereas fluorescence is not directly visible in a standard electron microscope, gold particles, as the most common choice of probe for EM can also only be visualised using specialised light microscopes.
In this review we will discuss some of the latest developments of probes for Correlative Light Electron Microscopy and some strategies how to choose a probe, discussing pros and cons of specific probes, and ensuring that they function as a dual modality marker.
| Original language | English |
|---|---|
| Pages (from-to) | 901-910 |
| Number of pages | 10 |
| Journal | Microscopy Research and Technique |
| Volume | 86 |
| Issue number | 8 |
| Early online date | 27 Feb 2023 |
| DOIs | |
| Publication status | Published - 26 Jul 2023 |
Bibliographical note
Funding Information:HT was supported by an EPSRC PhD studentship as part of the Bristol Centre for Functional Nanomaterials. We thank the excellent staff of the Wolfson Bioimaging Facility for their continuing support.
Funding Information:
HT was supported by an EPSRC PhD studentship as part of the Bristol Centre for Functional Nanomaterials. We thank the excellent staff of the Wolfson Bioimaging Facility for their continuing support.
Publisher Copyright:
© 2023 The Authors. Microscopy Research and Technique published by Wiley Periodicals LLC.