Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain

Cynthia L Andoniadou; Massimo Signore; Ezat Sajedi; Carles Gaston-Massuet; Daniel Kelberman; Alan J Burns; Nobue Itasaki; Mehul Dattani; Juan Pedro Martinez-Barbera

doi:10.1242/dev.02829

Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain

Cynthia L Andoniadou, Massimo Signore, Ezat Sajedi, Carles Gaston-Massuet, Daniel Kelberman, Alan J Burns, Nobue Itasaki, Mehul Dattani, Juan Pedro Martinez-Barbera

School of Anatomy

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Abstract

The homeobox gene Hesx1 is an essential repressor that is required within the anterior neural plate for normal forebrain development in mouse and humans. Combining genetic cell labelling and marker analyses, we demonstrate that the absence of Hesx1 leads to a posterior transformation of the anterior forebrain (AFB) during mouse development. Our data suggest that the mechanism underlying this transformation is the ectopic activation of Wnt/beta-catenin signalling within the Hesx1 expression domain in the AFB. When ectopically expressed in the developing mouse embryo, Hesx1 alone cannot alter the normal fate of posterior neural tissue. However, conditional expression of Hesx1 within the AFB can rescue the forebrain defects observed in the Hesx1 mutants. The results presented here provide new insights into the function of Hesx1 in forebrain formation.

Original language	English
Pages (from-to)	1499-1508
Number of pages	10
Journal	Development (Cambridge)
Volume	134
Issue number	8
Early online date	14 Mar 2007
DOIs	https://doi.org/10.1242/dev.02829
Publication status	Published - Apr 2007

Keywords

Animals
Axin Protein
Cell Differentiation
Cell Line
Cell Lineage
Cytoskeletal Proteins
Eye Proteins
Homeodomain Proteins
Mice
Mice, Knockout
Mutation
Nerve Tissue Proteins
Prosencephalon
Repressor Proteins
Transcription Factors
Wnt1 Protein
beta Catenin

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@article{32ae089778c24294a20da20584febd08,

title = "Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain",

abstract = "The homeobox gene Hesx1 is an essential repressor that is required within the anterior neural plate for normal forebrain development in mouse and humans. Combining genetic cell labelling and marker analyses, we demonstrate that the absence of Hesx1 leads to a posterior transformation of the anterior forebrain (AFB) during mouse development. Our data suggest that the mechanism underlying this transformation is the ectopic activation of Wnt/beta-catenin signalling within the Hesx1 expression domain in the AFB. When ectopically expressed in the developing mouse embryo, Hesx1 alone cannot alter the normal fate of posterior neural tissue. However, conditional expression of Hesx1 within the AFB can rescue the forebrain defects observed in the Hesx1 mutants. The results presented here provide new insights into the function of Hesx1 in forebrain formation.",

keywords = "Animals, Axin Protein, Cell Differentiation, Cell Line, Cell Lineage, Cytoskeletal Proteins, Eye Proteins, Homeodomain Proteins, Mice, Mice, Knockout, Mutation, Nerve Tissue Proteins, Prosencephalon, Repressor Proteins, Transcription Factors, Wnt1 Protein, beta Catenin",

author = "Andoniadou, {Cynthia L} and Massimo Signore and Ezat Sajedi and Carles Gaston-Massuet and Daniel Kelberman and Burns, {Alan J} and Nobue Itasaki and Mehul Dattani and Martinez-Barbera, {Juan Pedro}",

year = "2007",

month = apr,

doi = "10.1242/dev.02829",

language = "English",

volume = "134",

pages = "1499--1508",

journal = "Development (Cambridge)",

issn = "1477-9129",

publisher = "Company of Biologists Ltd",

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TY - JOUR

T1 - Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain

AU - Andoniadou, Cynthia L

AU - Signore, Massimo

AU - Sajedi, Ezat

AU - Gaston-Massuet, Carles

AU - Kelberman, Daniel

AU - Burns, Alan J

AU - Itasaki, Nobue

AU - Dattani, Mehul

AU - Martinez-Barbera, Juan Pedro

PY - 2007/4

Y1 - 2007/4

N2 - The homeobox gene Hesx1 is an essential repressor that is required within the anterior neural plate for normal forebrain development in mouse and humans. Combining genetic cell labelling and marker analyses, we demonstrate that the absence of Hesx1 leads to a posterior transformation of the anterior forebrain (AFB) during mouse development. Our data suggest that the mechanism underlying this transformation is the ectopic activation of Wnt/beta-catenin signalling within the Hesx1 expression domain in the AFB. When ectopically expressed in the developing mouse embryo, Hesx1 alone cannot alter the normal fate of posterior neural tissue. However, conditional expression of Hesx1 within the AFB can rescue the forebrain defects observed in the Hesx1 mutants. The results presented here provide new insights into the function of Hesx1 in forebrain formation.

AB - The homeobox gene Hesx1 is an essential repressor that is required within the anterior neural plate for normal forebrain development in mouse and humans. Combining genetic cell labelling and marker analyses, we demonstrate that the absence of Hesx1 leads to a posterior transformation of the anterior forebrain (AFB) during mouse development. Our data suggest that the mechanism underlying this transformation is the ectopic activation of Wnt/beta-catenin signalling within the Hesx1 expression domain in the AFB. When ectopically expressed in the developing mouse embryo, Hesx1 alone cannot alter the normal fate of posterior neural tissue. However, conditional expression of Hesx1 within the AFB can rescue the forebrain defects observed in the Hesx1 mutants. The results presented here provide new insights into the function of Hesx1 in forebrain formation.

KW - Animals

KW - Axin Protein

KW - Cell Differentiation

KW - Cell Line

KW - Cell Lineage

KW - Cytoskeletal Proteins

KW - Eye Proteins

KW - Homeodomain Proteins

KW - Mice

KW - Mice, Knockout

KW - Mutation

KW - Nerve Tissue Proteins

KW - Prosencephalon

KW - Repressor Proteins

KW - Transcription Factors

KW - Wnt1 Protein

KW - beta Catenin

U2 - 10.1242/dev.02829

DO - 10.1242/dev.02829

M3 - Article (Academic Journal)

C2 - 17360769

SN - 1477-9129

VL - 134

SP - 1499

EP - 1508

JO - Development (Cambridge)

JF - Development (Cambridge)

IS - 8

ER -

Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain

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Keywords

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