Lactose as a "trojan horse" for quantum dot cell transport

David Benito-Alifonso; Shirley Tremel; Bo Hou; Harriet Lockyear; Judith Mantell; David J Fermin; Paul Verkade; Monica Berry; M Carmen Galan

doi:10.1002/anie.201307232

Lactose as a "trojan horse" for quantum dot cell transport

David Benito-Alifonso, Shirley Tremel, Bo Hou, Harriet Lockyear, Judith Mantell, David J Fermin, Paul Verkade, Monica Berry^*, M Carmen Galan

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

61 Citations (Scopus)

Abstract

A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells. We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization. Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

Original language	English
Pages (from-to)	810-814
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	53
Issue number	3
DOIs	https://doi.org/10.1002/anie.201307232
Publication status	Published - 13 Jan 2014

Bibliographical note

Keywords

glycans
intracellular localization
multivalency
nanoparticles
quantum dots
GOLD NANOPARTICLES
PROTEIN
ENDOCYTOSIS
GLYCONANOPARTICLES
GLYCOBIOLOGY
NANOSCALE
MEDICINE
LIGANDS
BIOLOGY
FATE

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10.1002/anie.201307232

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Photo-oxidation and cryofluorescence for Correlative Light Electron Microscopy
Stephens, D. J. (Principal Investigator)
1/12/13 → 1/12/16
Project: Research
Reworking of Novel tools for Glycoscience
Galan, M. C. (Principal Investigator)
31/03/12 → 30/08/17
Project: Research
SUPERGEN EXITONIC SOLAR CELL CONSORTIUM - MAIN CORE
Fermin, D. J. (Principal Investigator)
1/04/09 → 1/04/13
Project: Research

Professor M C Galan
- M.C.Galanbristol.acuk
- School of Chemistry - Professor of Organic and Biological Chemistry
Person: Academic
Professor Paul Verkade
- P.Verkadebristol.acuk
- School of Biochemistry - Professor of Bioimaging
- Dynamic Cell Biology
Person: Academic , Member

Cite this

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title = "Lactose as a {"}trojan horse{"} for quantum dot cell transport",

abstract = "A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells. We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization. Moreover, we show that lactose can act as a {"}Trojan horse{"} on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.",

keywords = "glycans, intracellular localization, multivalency, nanoparticles, quantum dots, GOLD NANOPARTICLES, PROTEIN, ENDOCYTOSIS, GLYCONANOPARTICLES, GLYCOBIOLOGY, NANOSCALE, MEDICINE, LIGANDS, BIOLOGY, FATE",

author = "David Benito-Alifonso and Shirley Tremel and Bo Hou and Harriet Lockyear and Judith Mantell and Fermin, {David J} and Paul Verkade and Monica Berry and Galan, {M Carmen}",

note = "Copyright {\textcopyright} 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2014",

month = jan,

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doi = "10.1002/anie.201307232",

language = "English",

volume = "53",

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journal = "Angewandte Chemie - International Edition",

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T1 - Lactose as a "trojan horse" for quantum dot cell transport

AU - Benito-Alifonso, David

AU - Tremel, Shirley

AU - Hou, Bo

AU - Lockyear, Harriet

AU - Mantell, Judith

AU - Fermin, David J

AU - Verkade, Paul

AU - Berry, Monica

AU - Galan, M Carmen

PY - 2014/1/13

Y1 - 2014/1/13

N2 - A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells. We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization. Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

AB - A series of glycan-coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells. We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization. Moreover, we show that lactose can act as a "Trojan horse" on bi-functionalized QDs to help intracellular delivery of other non-internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway.

KW - glycans

KW - intracellular localization

KW - multivalency

KW - nanoparticles

KW - quantum dots

KW - GOLD NANOPARTICLES

KW - PROTEIN

KW - ENDOCYTOSIS

KW - GLYCONANOPARTICLES

KW - GLYCOBIOLOGY

KW - NANOSCALE

KW - MEDICINE

KW - LIGANDS

KW - BIOLOGY

KW - FATE

U2 - 10.1002/anie.201307232

DO - 10.1002/anie.201307232

M3 - Article (Academic Journal)

C2 - 24311369

SN - 1433-7851

VL - 53

SP - 810

EP - 814

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 3

ER -

Lactose as a "trojan horse" for quantum dot cell transport

Abstract

Bibliographical note

Keywords

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Projects

Photo-oxidation and cryofluorescence for Correlative Light Electron Microscopy

Reworking of Novel tools for Glycoscience

SUPERGEN EXITONIC SOLAR CELL CONSORTIUM - MAIN CORE

Profiles

Professor M C Galan

Professor Paul Verkade

Cite this